Abstract: HPLC fingerprinting and determination methods were applied to evaluate 88 batches of Rhizoma Coptidis from 27 different areas. The results showed that HPLC fingerprint of Rhizoma Coptidis was established with good separation and repeatability, which could be used for quality assessment of Rhizoma Coptidis. Twelve common peaks were defined in characteristic fingerprints, and similarity evaluation system was applied to evaluate the fingerprints of 88 batches of Rhizoma Coptidis, the contents of six alkaloids have been determined. The method of assessing Rhizoma Coptidis and evaluation of its quality has been established.

Key words: Rhizoma Coptidis, HPLC fingerprint, Identification, Quality