Anti-oxidative and anti-neuroinflammatory effects of corylin in H2O2-induced HT22 cells and LPS-induced BV2 cells by activating Nrf2/HO-1 and inhibiting NF-κB pathways

doi:10.5246/jcps.2023.02.007

Abstract

Abstract:

Oxidative damage and neuroinflammation are associated with numerous neurological diseases. In recent years, Psoraleae Fructus (dried fruits of Psoralea corylifolia L.), a traditional Chinese medicine, has been found to have medicinal properties in ameliorating central nervous system (CNS) injury. This study aimed to evaluate two neuroprotective compounds of Psoraleae Fructus: corylin and psoralidin, and reveal their underlying mechanisms. The results showed that corylin and psoralidin suppressed reactive oxygen species (ROS) production in H₂O₂-induced HT22 cells and nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV2 cells. Further mechanism research of corylin was carried out owing to its better safety and efficacy. In H₂O₂-induced HT22 cells, corylin significantly increased catalase (CAT), superoxide dismutase (SOD) activity, and glutathione (GSH) expression, while it inhibited mitochondrial membrane potential (MMP) reduction. Meanwhile, the expressions of Nrf2 and HO-1 were up-regulated. In LPS-induced BV2 cells, corylin markedly inhibited IL-1β, IL-6, and TNF-α expressions and specifically suppressed NF-κB p65 nuclear translocation. Furthermore, the docking analysis showed that corylin penetrated well into the hydrophobic pockets of Keap 1 and NF-κB protein. This study demonstrated that corylin alleviated oxidative damage and neuroinflammation by activating Nrf2/HO-1 and inhibiting NF-κB pathways, suggesting its potential ability to treat neurological diseases.

Key words: Corylin, Oxidative damage, Neuroinflammation, Nrf2, NF-κB

Supporting:

Zhaojing Wang, Qingxia Xu, Jing Xu, Wei Xu, Xiuwei Yang. Anti-oxidative and anti-neuroinflammatory effects of corylin in H2O2-induced HT22 cells and LPS-induced BV2 cells by activating Nrf2/HO-1 and inhibiting NF-κB pathways[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(2): 85-100.

Figures/Tables 9

References 46

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