Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques

doi:10.5246/jcps.2020.07.044

Abstract

Abstract:

In the present study, we aimed to assess the chemical composition changes of Semen Euphorbiae (SE) and Semen Euphorbiae Pulveratum (SEP) by UPLC-Q-TOF/MS and multivariate statistical methods. The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods. A “component-target-disease” network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine (TCM) to predict potential related diseases. The differences of chemical composition were significant between SE and SEP. Under positive ion mode, the amounts of Euphorbia factor L₂, L₃, L_7a, L₈, L₉ and lathyrol were obviously decreased after processing. Under negative ion mode, the amounts of aesculetin, bisaesculetin, ingenol and cetylic acid were increasedobviously, while Euphorbia factor L₁, L₄ and L₅ were decreased obviously after processing, and the components of euphobiasteroid, aesculetin, lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the “component-target-disease” network model. UPLC-Q-TOF/MS technology in combination with multivariate statisticalmethods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratumof SE. It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum, and laid the foundation for its further development and utilization.

Key words: Semen Euphorbiae, Semen Euphorbiae Pulveratum, UPLC-Q-TOF/MS, Multivariate statistical techniques, Chemical constituents, Manufacturing pulveratum

CLC Number:

R284

Supporting:

Huinan Wang, Jingzhen Zhang, Yuexin Cui, Siyu Wang, Hui Gao, Yao Zhang, Xinjie Wang, Ziye Yang, Mengyu Chen, Peihua Wang, Guimei Zhang, Yingzi Wang, Chao Zhang. Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(7): 470-479.

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