Chemical profiling of Scutellaria barbata by ultra high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry

doi:10.5246/jcps.2015.10.081

Abstract

Abstract:

An ultrahigh performanceliquid chromatographycoupled with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-quadrupole-orbitrap MS) method was developed to systematically analyze chemical constituents of Scutellaria barbata D. Don. The 70% methanol extract was separated on an Agilent Eclipse Plus C₁₈ column (1.8 μm, 150 mm×2.1 mm), and eluted with a gradient of methanol–acetonitrile–water containing 0.1% formic acid at a flow rate of 0.25 mL/min. Constituents were identified by HRMS in the negative ion mode using both full scan and two-stage threshold-triggered mass modes. A total of 56 compounds, including 20 free flavonoids, 20 flavonoid O-glycosides, 2 flavonoid C-glycosides, 4 phenylethanoid glycosides, and 10 phenolic compoundswere identified by analyzing their high resolution mass spectral data.Among them, 8 flavonoids and 2 phenylethanoid glycosides were unambiguously identified by comparing with reference standards. This study demonstrates that hybrid quadrupole-orbitrap mass spectrometry is a powerful tool in structural identification of unknown compounds in complicated herbal extracts.

Key words: Scutellaria barbata, Chemical analysis, Quadrupole-orbitrap mass spectrometry, Flavonoids

CLC Number:

R284

Supporting:

Ru Li, Wei Song, Xue Qiao, Jia Liu, Hong Liang, Min Ye . Chemical profiling of Scutellaria barbata by ultra high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 635-646.

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