Identification of major parent compounds and metabolites in bile, plasma and urine of rats after oral administration of Radix Scutellariae extract by UFLC-IT-TOF/MS

doi:10.5246/jcps.2013.04.046

Abstract

Abstract:

Flavonoids are the main bioactive components of Radix Scutellariae, a well-known traditional Chinese medicine. After oral administration of Radix Scutellariae extract (RSE), the bile, plasma and urine of rats were analyzed to identify the absorbed parent components and metabolites by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). A total of 36 different flavonoids, including 13 new metabolites, were identified or tentatively characterized in bile, plasma and urine of rats based on their fragmentation patterns. Among them, 16 flavonoids were identified in RSE in vitro, 25 flavonoids in rat bile, 15 flavonoids in rat plasma, and 14 flavonoids in rat urine. The results indicated that glucuronidation, sulfation and methylation were the major metabolic pathways of flavonoids. It is the first time that the metabolism of RSE was studied comprehensively.

Key words: Radix Scutellariae, Flavonoids, Metabolites, UFLC-IT-TOF/MS

CLC Number:

R917

Supporting:

Zhengwei Chen, Ling Tong, Shuming Li, Dongxiang Li, Xiaolin Liu, Li Ding^*, Yonghong Zhu, Shuiping Zhou, He Sun. Identification of major parent compounds and metabolites in bile, plasma and urine of rats after oral administration of Radix Scutellariae extract by UFLC-IT-TOF/MS[J]. , DOI: 10.5246/jcps.2013.04.046.

References

[1] Zhang, L.; Zhang, R.W.; Li, Q.; Lian, J.W.; Liang, J.; Chen, X.H.; Bi, K.S. Chromatographia. 2007, 66, 13-20.
[2] Li, B.Q.; Fu, T.; Gong, W.H.; Dunlop, N.; Kung, H.; Yan, Y.; Kang, J.; Wang, J.M. Immunopharmacology. 2000, 49, 295-306.
[3] Chi, Y.S.; Cheon, B.S.; Kim, H.P. Biochem. Pharmacol. 2001, 61, 1195-1203.
[4] Chen, Y.C.; Yang, L.L.; Lee, T.J.F. Biochem. Pharmacol. 2000, 59, 1445-1457.
[5] Ikemoto, S.; Sugimura, K.; Yoshida, N.; Yasumoto, R.; Wada, S.; Yamamoto, K.; Kishimoto, T. Urology. 2000, 55, 951-955.
[6] Ikemoto, S.; Sugimura, K.; Kuratukuri, K.; Nakatani, T. Anticancer Res. 2004, 24, 733-736.
[7] Sun, Y.; Lu, N.; Ling, Y.; Gao, Y.; Chen, Y.; Wang, L.; Hu, R.; Qi, Q.; Liu, W.; Yang, Y. Eur. J. Pharmacol. 2009, 603, 22-28.
[8] Evers, D.L.; Chao, C.F.; Wang, X.; Zhang, Z.; Huong, S.M.; Huang, E.S. Antiviral Res. 2005, 68, 124-134.
[9] Guo, Q.; Zhao, L.; You, Q.; Yang, Y.; Gu, H.; Song, G.; Lu, N.; Xin, J. Antiviral Res. 2007, 74, 16-24.
[10] Ma, S.C.; Du, J.; But, P.P.H.; Deng, X.L.; Zhang, Y.W.; Ooi, V.E.C.; Xu, H.X.; Lee, S.H.S.; Lee, S.F. J. Ethnopharmacol. 2002, 79, 205-211.
[11] de Boer, J.G.; Quiney, B.; Walter, P.B.; Thomas, C.; Hodgson, K.; Murch, S.J.; Saxena, P.K. Mutat. Res. Fundam. Mol. Mech. Mutagen. 2005, 578, 15-22.
[12] Zhang, D.Y.; Wu, J.; Ye, F.; Xue, L.; Jiang, S.; Yi, J.; Zhang, W.; Wei, H.; Sung, M.; Wang, W.; Li, X. Cancer Res. 2003, 63, 4037-4043.
[13] Shao, Z.H.; Hoek, T.L.V.; Li, C.Q.; Schumacker, P.T.; Becker, L.B.; Chan, K.C.; Qin, Y.; Yin, J.J.; Yuan, C.S. Am. J. Chin. Med. 2004, 32, 89-96.
[14] Tong, L.; Wan, M.; Zhang, L.; Zhu, Y.; Sun, H.; Bi, K. J. Pharm. Biomed. Anal. 2012, 70, 6-12.
[15] Wang, Y.; Yang, J.; Li, X.; Wang, J. Evid. Based Complement. Alternat. Med. 2012, 2012, ID 404529.
[16] Feng, N.P.; Di, B.; Liu, W.Y. Chem. Pharm. Bull. 2005, 53, 978-983.
[17] Chen, X.; Wang, H.; Du, Y.; Zhong, D. J. Chromatogr. B. 2002, 775, 169-178.
[18] Han, J.; Ye, M.; Xu, M.; Sun, J.; Wang, B.; Guo, D. J. Chromatogr. B. 2007, 848, 355-362.
[19] Wu, W.; Song, F.R.; Liu, Z.Q.; Liu, S.Y. Chem. J. Chin. Univ. 2005, 26, 27-30.
[20] Ferreres, F.; Silva, B.M.; Andrade, P.B.; Seabra, R.M.; Ferreira, M.A. Phytochem. Anal. 2003, 14, 352-359.
[21] Zhou, Q.; Zheng, Z.; Xia, B.; Tang, L.; Lv, C.; Liu, W.; Liu, Z.; Hu, M. Pharm. Res. 2010, 27, 1568-1583.
[22] Dittrich, P.; Ostrowski, J.; Beubler, E.; Schraven, E.; Kukovetz, W. Arzneimittelforschung. 1985, 35, 765-767.
[23] Zhang, W.; Saif, M.W.; Dutschman, G.E.; Li, X.; Lam, W.; Bussom, S.; Jiang, Z.; Ye, M.; Chu, E.; Cheng, Y.C. J. Chromatogr. A. 2010, 1217, 5785-5793.
[24] Hu, Y.; Jiang, P.; Wang, S.; Yan, S.; Xiang, L.; Zhang, W.; Liu, R. J. Ethnopharmacol. 2012, 141, 728-735.
[25] Liu, G.; Ma, J.; Chen, Y.; Tian, Q.; Shen, Y.; Wang, X.; Yao, S. J. Chromatogr. A. 2009, 1216, 4809-4814.
[26] Li, C.; Zhang, L.; Lin, G.; Zuo, Z. J. Pharm. Biomed. Anal. 2011, 54, 750-758.
[27] Guo, X.Y.; Han, J.; Ye, M.; Ma, X.C.; Shen, X.; Xue, B.B.; Che, Q.M. J. Pharm. Biomed. Anal. 2012, 63, 29-39.
[28] Li, S.L.; Song, J.Z.; Qiao, C.F.; Zhou, Y.; Xu, H.X. J. Pharm. Biomed. Anal. 2010, 52, 468-478.
[29] Qu, J.; Liang, Q.; Luo, G.; Wang, Y. Anal. Chem. 2004, 76, 2239-2247.
[30] Wen, H.Z. Nat. Prod. Res. Dev. 2004, 6, 575-580.