Abstract: Aim To study the chemical constituents of Hedysarum gmelinii. Methods The constituents were separated and purified by different methods of chromatography, and their structures were elucidated by IR, MS and NMR. Results Eight compounds were isolated from Hedysarum gmelinii, including three triterpenoids, two flavonoids and two other compounds. Their structures were identified as squasapogenol (1), soyasapogenol (2), lupeol (3), 3, 9-dihydroxy coumestan (4), 3-hydroxy-9-methoxy pterocarpan (5), β-sitosterol (6), palmatic acid (7), and hexadecanoic acid 2, 3-dihydroxypropyl ester (8). Conclusion All the compounds have been isolated from this plant for the first time. Compounds 1-4 and 8 were obtained from this genus for the first time. The NMR data of 1 are reported for the first time.

Key words: Hedysarum gmelinii, Hedysarum gmelinii, chemical constituents, chemical constituents, squasapogenol, squasapogenol