Abstract: Aim To establish a method for determination of Ginkgo biloba L, its extract and preparations with HPLC fingerprints, so as to c ontrol the quality of the preparations. Methods HPLC-DAD method was used to determine the constituents in preparations. Diamonsil ^TM C18(200 mm×4.6 mm, 5 μm) was used as analytical column, and acetonitrile/KH2PO4 was used as mobile phase with gradient elution. The column temperatu re was at 24 ºC. The HPLC profile of chemical constituents of control sample and preparations were analyzed using similarity software. Results The fingerprints of different preparations from different companies were slightl y different because of the different preparing procedures. Mean while, the finge rprints of different batches of the same preparation from the same company were similar to each other and the technology of each preparation was stable. Conclusion This method is accurate, reproducible, simple, and can be used as an analytical method for the routine quality control of Ginkgo biloba preparations.

Key words: Ginkgo biloba, Ginkgo biloba, high-performance liquid chroma tography, high-performance liquid chroma tography, fingerprints, fingerprints, similarity, similarity