Determination of Eleutheroside B and E in Acanthopanax Preparations by High-Performance Liquid Chromatography with Solid-Phase Extraction

Abstract

Abstract: Aim An HPLC method for analyzing eleutheroside B (ELU B) and eleutheroside E (ELU E), two of the main active substances of Acanthopanax preparations were studied. Methods The samples were analyzed on a kromasil ODS column with water-acetonitrile as a gradient mobile phase. The flow rate was 0.8 mL·min-1 and detecting wavelengths were 206 nm for ELU B, 220 nm for ELU E, solid phase extraction (SPE) and internal standard-salicin were selected. Results The recoveries of Acanthopanax tablets and injection were 90.4%-96.8% and 96.4%-99.8% for ELU B, 87.7%-93.3% and 95.7%-98.5% for ELU E, respectively. The linear ranges were 4.45-22.25 μg·mL^-1(r = 0.9998) and 5.11-25.55 μg·mL^-1(r = 0.9997) respectively. Conclusion This method can save the time for cleaning the chromatographic system and improve sensitivity for Acanthopanax preparations, thus providing a way to evaluate the quality of Acanthopanax preparations.

Key words: eleutherosides, eleutherosides, Acanthopanax preparations, Acanthopanax preparations, HPLC, HPLC, solid phase extraction, solid phase extraction

CLC Number:

R917.01

R917.792

Supporting:

HU Fang-di, FENG Shi-lan^*, ZHAO Jian-xiong, CHEN Li-ren, XU Jing-wen. Determination of Eleutheroside B and E in Acanthopanax Preparations by High-Performance Liquid Chromatography with Solid-Phase Extraction[J]. .

References

[1] Xu GJ. Pharmacognosy [M]. 2nd ed. Beijing : People’s Health Publishing House, 1996, 389-390.
[2] Jiangsu Medical College. Dictionary of Traditional Chinese Medicine [M]. Shanghai : Shanghai Scientific and Technical Publishing House, 1995, 380-382.
[3] Sandberg F. Two glycoside-containing genera of the Araliaceae family, Panax and Eleutherococcus[J]. Planta Med, 1973, 24 (1) : 392-396.
[4] Barenboim GM, Brikenshtein VKh, Shul’man ML, et al. Luminescence analysis in pharmacological studies. III. Fluorescence of individual eleutheroside and an extract of Eleutherococcus [J]. Khim Farm Zh, 1984, 18 (4) : 493-500.
[5] Todorov IN, Sizova ST, Mitrokhin YI, et al. Study on the pharmacokinetics and mechanism of action of Eleutherococcus glycosides. VII. Effect of the extract and of individual glucosides on protein biosynthesis [J]. Khim Farm Zh, 1984, 18 (8) : 920-924.
[6] Takasugi N, Moriguchi T, Fuwa T, et al. Effect of Eleutherococcus senticosus on rectal temperature, body and grip tones, mortor coordination, and exploratory and spontaneous movement in acute stressed mice [J]. Shoyakugaku Zasshi, 1985, 39 (3) : 232-237.
[7] Solo’eva AG, Petukhova TV, Greshnykh RD. Analysis and standardization of Eleutherococcus roots and rhizomes and liquid extracts according to the content of biologically active compounds [J]. Farmatsiya, 1989, 38 (1) : 25-27.
[8] Jong SK, Pham TL, Xing FC, et al. Quantitative determination of ELU B and E from Acanthopanax species by high performance liquid chromatography [J]. Arch Pharm Res, 2001, 24 (5) : 407-411.
[9] Feng SL, Chen LR, Zhao FH, et al. Determination of eleutherosides B and Eof Acathopanax by HPLC [J]. Chin J Mod Appl Pharm, 2001, 18 (2) : 124-126.
[10] State Pharmacopoeia Committee of People’s Republic of China. Pharmacopoeia of the People’s Republic of China [S]. 2000 Ed. Beijing : Chemical Industry Press, 2000. 491.
[11] State Pharmacopoeia Committee of People’s Republic of China. Pharmacopoeia of the People’s Republic of China [S]. The Drug Standards Issned by the Ministry of Health, People’s Republic of China. 1998, Vol 18. 162-163.
[12] Wu YC, Huang SD. Solid-phase microextraction coupled with High-performance liquid chromatography for the determination of aromatic amines [J]. Anal Chem, 1999, 71 (2) : 310-318.
[13] Chen J, Pawliazyn JB. Solid phase microextraction coupled with high-performance liquid chromatography [J]. Anal Chem, 1995, 67 (5) : 2530-2533.
[14] Liu LC, Zhao FH, Chen LR. Determination of content of eleutherosides B and E in Acanthopanax by HPLC [J]. Anal Test Technol Instrum, 2000, 6 (2) : 93-94.
[15] Wang J, Liu LC. Determination of eleutherosides B, E of Acanthopanax extract by HPLC [J]. Lishizhen Medic Mat Med Res, 2003, 14 (7) : 398-399.