Abstract: Aim To develop a rapid, effective method for the determination of flavonoids and ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to control the ratio of Epimedium and Ginseng herbs in botanicalcombinations. Methods The quality evaluation was determinatted using reversed-phase high performance liquid chromatog raphy (HPLC), referred by the major flavonoids from Epimedium, epimedin A, epimedin B, epimedin C, and icariin as thestandards, and the major ginsenosides Rg1 , Re, Rf, Rb1 , Rb2, and Rd as the standards, included Epimedium brevicornum Maxim., E. sagittatum (Sieb. et Zucc) Maxim., E. koreanum NaKai, P. ginseng C. A. Meyer, P. quinquefolium L., P. notoginseng and some products containing the above herbs. Results The main flavonoids and ginsenosides couldbe clearly resolved in the single analysis. Conclusion The results can be effectively used in evaluating qualitatively andquantitatively the ration of Epimedium and Ginseng contained products.

Key words: epimedium, epimedium, ginseng, ginseng, flavonoids, flavonoids, ginsenosides, ginsenosides, HPLC-MS analysis., HPLC-MS analysis.