Fingerprint analysis of Ophiopogonis Radix by HPLC-UV-ELSD coupled with chemometrics methods

doi:10.5246/jcps.2013.01.007

Abstract

Abstract:

A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA). The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopogonis Radix.

Key words: Ophiopogonis Radix, Ophiopogon japonicus, HPLC-UV-ELSD, Fingerprint, Chemometrics, Quality evaluation

CLC Number:

R284

Supporting:

Cite this article

Ning Li, Yanyun Che, Liang Zhang, Jiayu Zhang, Yuan Zhou, Yong Jiang, Pengfei Tu^*. Fingerprint analysis of Ophiopogonis Radix by HPLC-UV-ELSD coupled with chemometrics methods [J]. , DOI: 10.5246/jcps.2013.01.007.

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URL: http://jcps.bjmu.edu.cn/EN/10.5246/jcps.2013.01.007

http://jcps.bjmu.edu.cn/EN/Y2013/V22/I1/55

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