抗坏血酸与丝裂霉素C对艾氏腹水癌细胞联合杀伤作用的体外研究

抗坏血酸与丝裂霉素C对艾氏腹水癌细胞联合杀伤作用的体外研究

闵熙, 李泮海, 庞贻慧

1. 北京同仁医院药剂科, 北京 100005;
2. 北京医科大学药学院物理化学教研室, 北京 100083

收稿日期:1994-01-08 修回日期:1994-11-08 出版日期:1995-09-15 发布日期:1995-09-15

A Study in vitro on the Synergetic Cytocidal Effect of Ascorbic Acid and Mitomycin C against the Ehrlich As-cites Tumor Cells in Culture

Xi Min, Pan-Hai Li, Yi-Hui Pang

1. Beijing Tong Ren Hospital; Beijing 100005;
2. Department of Physical Chemistry; School of Pharmacy; Beijing Medical University; Beijing 100083

Received:1994-01-08 Revised:1994-11-08 Online:1995-09-15 Published:1995-09-15

摘要/Abstract

摘要： 研究了抗坏血酸与丝裂霉素C (Mitomycin C）对艾氏腹水癌细胞的联合杀伤作用, Cu(II)/ mitomycin C /抗坏血酸体系对DNA链的断裂作用, 以及丝裂霉素Ｃ存在下, Cu(II)催化抗坏血酸有氧氧化的动力学。实验结果表明, 抗坏血酸与丝裂霉素C在杀伤艾氏腹水癌细胞时存在协同作用, 该协同作用与Cu(II)/mitomycin C/抗坏血酸体系对DNA链的断裂作用以及Cu(II)/mitomycin C/抗坏血酸体系产生·OH自由基的量和速度有关, 其均与抗坏血酸浓度成正比。此结果提示, 抗坏血酸有氧氧化产生的·OH, 以及由之引起的DNA链断裂作用, 是导致抗坏血酸与丝裂霉素Ｃ在杀伤艾氏腹水癌细胞时存在协同作用的重要原因。

关键词: 坏血酸, 丝裂霉素C, 艾氏腹水癌细胞, DNA链的断裂作用

Abstract: The synergetic cytocidal effect of ascorbic acid and mitomycin C on Ehrlich as-cites tumor cells in culture was studied. The chain scission of DNA by Cu(II) mitomycin C/ascorbic acid system, and the chemical kinetics of the Cu(II) catalyzed aerobic oxidation of ascorbic acid in the presence of mitomycin C were also discussed. Experimental results showed that ascorbic acid and mitomycin C were synergetic in destroying Ehrlich ascites tumor cells.The effect is related to the breaking action of the Cu(II) mitomycin C/ascorbic acid system on the DNA chain, and to the quantity of the system produced ·OH, and the speed of production which are both directly proportional to the concentration of ascorbic aci.These results imply that the ·OH produced in the aerobic oxidation of ascorbic acid, and·OH induced scission of DNA chain, are important factors to he synergetic cytocidal effect of ascorbic acid and mitomycin C on Ehrlich ascites tumor cells.

Key words: Ascorbic acid, Mitomycin C, Ehrlich ascites tumor cells, Chain scission of

Supporting:

闵熙, 李泮海, 庞贻慧. 抗坏血酸与丝裂霉素C对艾氏腹水癌细胞联合杀伤作用的体外研究[J]. .

Xi Min, Pan-Hai Li, Yi-Hui Pang. A Study in vitro on the Synergetic Cytocidal Effect of Ascorbic Acid and Mitomycin C against the Ehrlich As-cites Tumor Cells in Culture[J]. .