用牛免疫缺陷病毒筛选抗抗艾滋病药物的实验模型

用牛免疫缺陷病毒筛选抗抗艾滋病药物的实验模型

王岱, 刘淑红, 陈启民, 耿运琪, 徐为人, 魏月芳

1. 南开大学生命科学学院, 天津 300071;
2. 国家医药管理局天津药物研究院, 天津 300193

收稿日期:1996-04-25 修回日期:1997-01-02 出版日期:1997-03-15 发布日期:1997-03-15

An Experimental Model for Screening Anti-AIDS Drugs with Bovine Immunodeficiency Virus

Dai Wang, Shu-Hong Liu, Qi-Min Chen, Yun-Qi Geng, Wei-Ren Xu, Yue-Fang Wei

1. College of Life Sciences; Nankai University; Tianjin 300071;
2. Tianjin Institute of Pharmaceutical Research; SPAC; Tianjin 300193

Received:1996-04-25 Revised:1997-01-02 Online:1997-03-15 Published:1997-03-15

摘要/Abstract

摘要： 本文应用牛免疫缺陷病毒(BIV)合胞体形成和BIV长末端重复序列(LTR)引导的萤火虫荧光素酶(Luc)基因表达分析, 建立了抗艾滋病(AIDS)药物的筛选和评价模型。BIV合胞体形成及BIV-LTR引导的Luc基因活力与BIV感染的细胞呈线性关系。临床有效药物3'-叠氮胸嘧啶脱氧核苷(AZT)可显著抑制BIV合胞体的形成及BIV-LTR引导的Luc基因表大家水平, 并有剂量依赖关系。上述两种分得到AZT的IC50分别为0.24和0.052 mmol/L。

关键词: 艾滋病, 药物筛选, 牛免疫缺陷病毒, 合胞体, 长末端重复序列, 3'-叠氮胸嘧啶脱氧核苷

Abstract: The assays for bovine immunodeficiency virus (BIV) induced syncytium formation and BIV long terminal repeat (LTR) directed luciferase (Luc) gene expression were applied to screen and evaluate anti AIDS drugs. Frequency of the syncytium formation and BIV LTR directed Luc activity were in proportion to the number of input BIV infected cells. AZT inhibited the syncytium formation and the BIV LTR directed Luc gene expression level. Its inhibitory effects were dosedependent with the IC50 being 0.24 and 0.052 mmol/L, respectively.

Key words: Acquired immunodeficiency syndrome (AIDS), Drug screening, Bovine immunodeficiency virus (BIV), Syncytium, Long terminal repeat (LTR), 3'-Azido-2',3'-dide-oxythymidine (AZT)

Supporting: First published in Chinese in Chinese Journal of Pharmacology and Toxicology 1996, 10 (1): 49-52.

王岱, 刘淑红, 陈启民, 耿运琪, 徐为人, 魏月芳. 用牛免疫缺陷病毒筛选抗抗艾滋病药物的实验模型[J]. .

Dai Wang, Shu-Hong Liu, Qi-Min Chen, Yun-Qi Geng, Wei-Ren Xu, Yue-Fang Wei. An Experimental Model for Screening Anti-AIDS Drugs with Bovine Immunodeficiency Virus[J]. .

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