Tween 胶束体系中水杨酸铜络合物的SOD样活性

Tween 胶束体系中水杨酸铜络合物的SOD样活性

刘艳^*, 张学东, 马思谦, 鲁纯素

北京医科大学药学院, 北京 100083

收稿日期:1996-04-11 修回日期:1996-12-05 出版日期:1997-03-15 发布日期:1997-03-15
通讯作者: 刘艳*

SOD like Activity of Copper Salicylate Complex in Tween Micellar Systems

Yan Liu^*, Xue-Dong Zhang, Si-Qian Ma, Chun-Su Lu

School of Pharmaceutical Sciences; Beijing Medical University; Beijing 100083

Received:1996-04-11 Revised:1996-12-05 Online:1997-03-15 Published:1997-03-15
Contact: Yan Liu*

摘要/Abstract

摘要： 合成了水杨酸铜络合物并分析了其组成, 提纯了Tween-20,-40,-60,-80, 并测定了它们在pH 7.4的磷酸缓冲液中的CMC。用Cyt c还原法分别测定了水杨酸铜络合物在不同介质中的SOD样活性。详细研究了表面活性剂与水杨酸铜络合物协同清除O2^-的作用。观察到在胶束体系中水杨酸铜络合物的SOD样活性明显高于缓冲液体系无表面活性剂时的SOD样活性, 表面活性剂本身也具有清除O2^-的功能。抗红细胞膜脂质过氧化实验也得到类似结果。这可用胶束催化解释。水杨酸铜-Tween体系可能是一种潜在的抗炎药物。

关键词: 超氧阴离子自由基, 羟自由基, 胶束催化, SOD样活性, 铜络合物

Abstract: Copper salicylate complex has been synthesized and its composition was analyzed. The four surfactants (Tween 20, 40, 60, 80) were purified and their critical micelle concentrations(CMC) in phosphate buffer (pH = 7.4) were measured. The SOD like activity of copper salicylate complex was assayed using the cytochrome creduction method in different media. The cooperative effect of the complex with micelles was studied in detail. SOD like activity of the complex was enhanced in the presence of surfactants. The four surfactants themselves display SOD like activities to a certain extent. Similar results were obtained in the experiment for the antilipid peroxidation of red cell membrane. These results might be explained by the fact of micellar catalysis. It was suggested that the copper salicylate Tween system used as a potential antiinflammatory drug might be worthwhile to be further studied.

Key words: Superoxide anion radical, Hydroxide radical, Micellar catalysis, SOD like activity, Copper complex

Supporting: The project is supported by the Natural Science Foundation of China.

刘艳^*, 张学东, 马思谦, 鲁纯素. Tween 胶束体系中水杨酸铜络合物的SOD样活性[J]. .

Yan Liu^*, Xue-Dong Zhang, Si-Qian Ma, Chun-Su Lu. SOD like Activity of Copper Salicylate Complex in Tween Micellar Systems[J]. .

[1]	刘艳, 贾彩, 侯新朴. 脂质体的电性对水杨酸铜SOD样活性的影响[J]. , 2001, 10(2): 94-96.
[2]	应宇辉, 张颖, 鲁纯素. 胶束体系中的水杨酸铜络合物对培养的巨噬细胞释放的超氧离子的清除作用[J]. , 1995, 4(1): 32-36.
[3]	刘雨, 张均田. 丹参酚酸类对羟自由基的捕捉作用[J]. , 1994, 3(1): 43-50.
[4]	史录文, 鲁纯素, 郑俊华, 楼之岑. 大黄十五种化合物的SOD样活性研究[J]. , 1992, 1(2): 59-64.