补阳还五汤及其有效成分对脑中风细胞膜流动性的影响

补阳还五汤及其有效成分对脑中风细胞膜流动性的影响

卢景芬, 李晨旭, 古力努尔, 李廷凤, 屠鹏飞, 殷俊杰, 蔡少青^*

1.北京大学医学部天然药物及仿生药物国家重点实验室, 北京100083;
2.美国FDAHFS717, 5100PaintBranchPkwy, CollegePark, MD20740, USA

收稿日期:2002-04-09 修回日期:2002-09-26 出版日期:2002-12-15 发布日期:2002-12-15
通讯作者: 蔡少青*

Effects of BYHW Decoction and Its Effective Constituents on the Fluidity of the Cell Membrane in a Stroke-Modeled Rat Brain

Lu Jingfen, Li Chenxu , Muteliefu Gulinuer, Li Tingfeng, Tu Pengfei, Yin Junjie, Cai Shaoqing^*

1.State Key Laboratory of Natural and Biomimetic Drugs, Health Science Center of Peking University, Beijing 100083;
2.Instrumentation and Biophysics Branch, CFSAN, FDA. 5100 Paint Branch Pkwy, College Park, MD20740 USA.

Received:2002-04-09 Revised:2002-09-26 Online:2002-12-15 Published:2002-12-15
Contact: Cai Shaoqing*

摘要/Abstract

摘要： 本文采用先进的自旋标记电子顺磁共振方法研究了补阳还五汤及其有效成分黄芪甲苷、阿魏酸、川芎嗪、芦丁、绿原酸、紫檀烷苷、毛蕊异黄酮、芒柄花素、毛蕊异黄酮苷、芍药苷、丹皮酚和槲皮素体外给药对颈总动脉结扎和释放造成缺血——再灌损伤的大鼠脑细胞流动性的影响。该损伤大鼠用作脑中风模型, 结果表明损伤大鼠脑细胞流动性较正常大鼠降低约8% , 而补阳还五汤及其有效成分对正常细胞无影响但可使损伤大鼠脑细胞膜流动性增大, 不同程度地恢复到接近于正常细胞膜流动性水平。本实验表明: 细胞膜流动性是一个敏感而重要的生物学指标, 反映细胞的活性状态。可用这一新的技术方法来研究补阳还五汤及其组方配伍的分子机理.

关键词: 补阳还五汤, 脑中风, 细胞膜流动性, 电子顺磁共振

Abstract: With in vitro spin labeling electron spin resonance (ESR) spectroscopy, we have studied the effects of Bu Yang Huan Wu (BYHW) decoction and its effective constituents such as astragaloside IV ferulic acid, chuanxiongzine, rutin, chlorogenic acid, 9,10 dimethoxy pterocarpane-7-O-β-D-glucoside, calycosin, formononetin, calycosin-7-O-glucoside, paeoniflorin, paeonal and quercein on the cell membrane fluidity of a rat brain which was modeled after the dual cervical arteries were intercepted and released for realizing an ischemia reperfusion injury which was selected as a brain stroke model. Our results indicated that the cell membrane fluidity in the model group decreased approximately 8% compared with the control group, and after brain cells were incubatied with species, the membrane fluidity could be recovered closely to the control level depending on the BYHW decoction and its different constituents. As the membrane fluidity is a very sensitive biological index which reflectsd the cell status, our method will be useful to study the molecular mechanism of tradition Chinese medicine (TCM) and its combination recipe.

Key words: Bu Yang Huan Wu decoction, Bu Yang Huan Wu decoction, Brain stroke, Brain stroke, Cell membrane fluidity, Cell membrane fluidity, ESR, ESR

Supporting: ^*Supported by State 95 project.

卢景芬, 李晨旭, 古力努尔, 李廷凤, 屠鹏飞, 殷俊杰, 蔡少青^*. 补阳还五汤及其有效成分对脑中风细胞膜流动性的影响[J]. .

Lu Jingfen, Li Chenxu , Muteliefu Gulinuer, Li Tingfeng, Tu Pengfei, Yin Junjie, Cai Shaoqing^*. Effects of BYHW Decoction and Its Effective Constituents on the Fluidity of the Cell Membrane in a Stroke-Modeled Rat Brain[J]. .

[1]	王圣鑫, 闫向丽, 郑昊圳, 余健烨, 余爱明, 沈晓, 王利胜. 微透析技术结合液质联用的不同剂量黄芪的补阳还五汤在脑缺血损伤大鼠脑药动学研究[J]. 中国药学(英文版), 2020, 29(2): 90-101.
[2]	许迎利, 白光灿, 卢景雰. 高校仪器共享平台电子顺磁共振波谱仪的管理[J]. 中国药学(英文版), 2019, 28(7): 519-526.
[3]	盛伊娜, 王申林, 刘国全. pH敏感的抗菌肽LAH4与膜相互作用的电子顺磁共振研究[J]. 中国药学(英文版), 2019, 28(11): 749-759.