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重组E.coli L-天冬酰胺酶在大鼠中的药物代谢动力学

陈建华, 吴梧桐*, 平野和行   

  1. 1.中国药科大学分子生物学教研室, 南京 210009, 中国;
    2.岐阜药科大学药剂学实验室, 岐阜 502-8585, 日本
  • 收稿日期:2002-06-11 修回日期:2002-10-15 出版日期:2002-12-15 发布日期:2002-12-15
  • 通讯作者: 吴梧桐*

Pharmacokinetics of Recombinant E. coli L-asparaginase in Rats

Chen Jianhua, Wu Wutong*, Hirano Kazuyuki

  

  1. 1. Department of Molecular Biology, China Pharmaceutical University, Nanjing 210009;
    2. Laboratory of Pharmaceutics, Gifu Pharmaceutical University, Gifu 502-8585 Japan
  • Received:2002-06-11 Revised:2002-10-15 Online:2002-12-15 Published:2002-12-15
  • Contact: Wu Wutong*

摘要: 应用放射性核素示踪技术研究12 5I重组E .coli L-天冬酰胺酶在大鼠体内的药物代谢动力学。125I重组L-天冬酰胺酶静脉注射后2 4小时内, 在尿, , 胆汁中的排泄量分别占注射剂量的68.95%, 4.44%5.36%。测定血浆中12 5I重组E. coli L-天冬酰胺酶浓度, 应用聚丙烯酰胺凝胶电泳和生物成像分析系统结合方法评价原药水平, 由房室模型评价药物动力学参数, 静注后, 浓度时间曲线符合二房室模型. 初期和末端的t1/2分别为0.52-0.63小时和2.39-2.76小时. AUC与剂量成正比。重组E .coli L-天冬酰胺酶的分解代谢产物主要随尿液排泄, 重组E .coli L-天冬酰胺酶在大鼠中的药物动力学参数为临床试验提供了有用依据.

关键词: 重组E. coli L-天冬酰胺酶, 药代动力学

Abstract: The distribution of 125I recombinant E .coli L-asparaginase in tissues or organs and the excretion in urine, feces and bile were studied with in vivo radioactive tracer technique. The amount of radioactivity excreted in urine, feces and bile within 24 h after intravenous administration of 125I recombinant E .coli L-asparaginase to rats was 68.95%,4.44% and 5.36% of the dose respectively.125I recombinant E .coli L-asparaginase in plasma samples was determined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGE and bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model dependent method. The concentration time curves of recombinant E .coli L-asparaginase after intravenous injection at 1250 IU·kg-1, 2500 IU·kg-1, 5000 IU·kg-1 to rats were consistent with the two compartment model. The first and terminal elimination t1/2 were 0.52~0.63h and 2.39~2.76h respectively. AUC was linearly related to the doses. The results of distribution in tissues or organs and excretion in urine suggested that the metabolites of the enzyme were cleared by mechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E .coli L asparaginase in rats are warranted for the design of future clinical trials.

Key words: Pecombinant E.coli L-asparaginase, Pecombinant E.coli L-asparaginase, Pharmacokinetics, Pharmacokinetics

Supporting: *Project supported by the National Ninth-Five Plan Key Project Foundation , No 96-902-01-25.