灵芝对动物胶原蛋白性关节炎的作用

灵芝对动物胶原蛋白性关节炎的作用

力弘, 贾永锋, 森昌夫, 张罗修

1. 上海医科大学药学院药理教研室, 上海 200032;
2. 日本和汉生药研究所, 东京

收稿日期:1999-05-17 修回日期:1999-09-28 出版日期:2000-03-15 发布日期:2000-03-15

Effect of Ganoderma lucidum on Collagen-Induced Arthritis in Rats

Li Hong, Jia Yongfeng, Masao MORI, Zhang Luoxiu

1. Department of Pharmacology School of pharmacy Shanghai Medical University Shanghai 200032;
2. Wakan Shoyaku Botany Institute. Tokyo. Japan

Received:1999-05-17 Revised:1999-09-28 Online:2000-03-15 Published:2000-03-15

摘要/Abstract

摘要： 本文观察了灵芝对大鼠胶原蛋白性关节炎的作用。II型胶原蛋白(CII)1.5mg/只d0背部皮内注射,引发胶原蛋白性关节炎(CIA)。酶联免疫吸附法测定血清抗CII抗体水平; [3H]-TdR掺入法测定鼠腹腔巨噬细胞产生白细胞介素-1(IL-1)的活性; 四甲基偶氮唑盐(比色法)测定鼠脾淋巴细胞培养上清中白细胞介素-2(IL-2)的活性。观测针对CII的皮肤迟发型变态反应和大鼠后足足肿改变水平。灵芝0.5 g·kg^-1和1.0 g·kg^-1·d^-1可抑制迟发型变态反应,灵芝1.0 g·kg^-1·d^-1可降低血清抗CII抗体水平, 恢复IL-2产生水平, 但对大鼠后足足肿以及白细胞介素-1(IL-1)产生无明显改善作用。提示灵芝作为免疫调节剂, 对大鼠CIA的抗炎效果较弱,但对免疫异常指标具有改善作用。

关键词: 灵芝, 胶原蛋白件关节炎, 抗Ⅱ型胶原蛋白抗体, 迟发型变态反应, 白细胞介素-1, 白细胞介素-2

Abstract: Collagen type II (CII) 1.5 mg was injected intradermally on d0 to induce arthritis (CIA) in rats. Hind paw volumes of rats were measured with a water displacement method. The serum anti-collagen antibody was measured by an enzyme-linked immunosorbent assay. Delayed hypersensitivity was reflected by skin response to CII. Interleukin-1 (IL-1) activities were evaluated by [3H]-TdR uptake and interleukin-2 (IL-2) activity were measured by MTT colorimetric assay. Ganoderma lucidum (leyss. ex Fr.) Karst. (GL) 0.5 and 1.0 g.kg^-1·d^-1, given iP to rats from d0, to d24, suppressed skin response to CII, 1.0 g.kg^-1 GL reduced high level of anti-collagen antibody and improved the lowered IL-2 production in CIA rats. GL had no significant effect on IL-1 production and could not reduce inflammatory swelling of hind paw in CIA rats. As an immunomodulator, GL had shown no significant antiinflammatory activity, but improved the abnormal immune function in CIA.

Key words: Collagen induced arthritis, Collagen induced arthritis, Anti-type II collagen antibody, Anti-type II collagen antibody, Ganoderma lucidum leyss ex Fr Karst, Ganoderma lucidum leyss ex Fr Karst, Delayed-type hypersensitivity, Delayed-type hypersensitivity, Interleukin-1, Interleukin-1, Interleukin-2, Interleukin-2

Supporting:

力弘, 贾永锋, 森昌夫, 张罗修. 灵芝对动物胶原蛋白性关节炎的作用[J]. .

Li Hong, Jia Yongfeng, Masao MORI, Zhang Luoxiu. Effect of Ganoderma lucidum on Collagen-Induced Arthritis in Rats[J]. .

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