刺五加制剂用固相萃取后以高效液相色谱法测定刺五加苷B、苷E的含量

刺五加制剂用固相萃取后以高效液相色谱法测定刺五加苷B、苷E的含量

胡芳弟, 封士兰^*, 赵健雄, 陈立仁, 徐静汶

1.兰州医学院, 甘肃兰州 730000;
2.中国科学院兰州化学物理研究所, 甘肃兰州 730000

收稿日期:2004-09-20 修回日期:2005-02-10 出版日期:2005-03-15 发布日期:2005-03-15
通讯作者: 封士兰*

Determination of Eleutheroside B and E in Acanthopanax Preparations by High-Performance Liquid Chromatography with Solid-Phase Extraction

HU Fang-di, FENG Shi-lan^*, ZHAO Jian-xiong, CHEN Li-ren, XU Jing-wen

1. Lanzhou Medical College, Lanzhou 730000, China;
2. Lanzhou Institute of Ch emical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

Received:2004-09-20 Revised:2005-02-10 Online:2005-03-15 Published:2005-03-15
Contact: FENG Shi-lan*

摘要/Abstract

摘要： 目的用高效液相色谱法研究刺五加制剂中2个主要活性成分刺五加苷B、苷E的含量测定方法.方法 Kromasil ODS柱, 水-乙腈梯度流动相, 流速0.8 mL·min^-1, 测定波长刺五加苷B 206 nm,刺五加苷E 220 nm, 水杨酸作为内标, 选择了固相萃取条件. 结果刺五加片中刺五加苷B、苷E的回收率范围分别是90.4%~96.8%和87.7%~93.3%; 刺五加注射液中刺五加苷B、苷E的回收率范围分别是96.4%~99.8%和95.7%~98.5%.线性范围分别是4.45~22.25 μg·mL^-1 (r = 0.9998)和5.11~25.55 μg·mL^-1 (r = 0.9997). 结论该方法节约了清洗色谱系统的时间, 提高了测定的灵敏度,提供了评价刺五加制剂质量的方法.

关键词: 刺五加苷, 刺五加苷, 刺五加苷, 刺五加制剂, 刺五加制剂, 刺五加制剂, 高效液相色谱, 高效液相色谱, 高效液相色谱, 固相萃取, 固相萃取, 固相萃取

Abstract: Aim An HPLC method for analyzing eleutheroside B (ELU B) and eleutheroside E (ELU E), two of the main active substances of Acanthopanax preparations were studied. Methods The samples were analyzed on a kromasil ODS column with water-acetonitrile as a gradient mobile phase. The flow rate was 0.8 mL·min-1 and detecting wavelengths were 206 nm for ELU B, 220 nm for ELU E, solid phase extraction (SPE) and internal standard-salicin were selected. Results The recoveries of Acanthopanax tablets and injection were 90.4%-96.8% and 96.4%-99.8% for ELU B, 87.7%-93.3% and 95.7%-98.5% for ELU E, respectively. The linear ranges were 4.45-22.25 μg·mL^-1(r = 0.9998) and 5.11-25.55 μg·mL^-1(r = 0.9997) respectively. Conclusion This method can save the time for cleaning the chromatographic system and improve sensitivity for Acanthopanax preparations, thus providing a way to evaluate the quality of Acanthopanax preparations.

Key words: eleutherosides, eleutherosides, Acanthopanax preparations, Acanthopanax preparations, HPLC, HPLC, solid phase extraction, solid phase extraction

中图分类号:

R917.01

R917.792

Supporting: ^*Corresponding author. Tel.: 86-931-8289515

胡芳弟, 封士兰^*, 赵健雄, 陈立仁, 徐静汶. 刺五加制剂用固相萃取后以高效液相色谱法测定刺五加苷B、苷E的含量[J]. .

HU Fang-di, FENG Shi-lan^*, ZHAO Jian-xiong, CHEN Li-ren, XU Jing-wen. Determination of Eleutheroside B and E in Acanthopanax Preparations by High-Performance Liquid Chromatography with Solid-Phase Extraction[J]. .

参考文献

[1] Xu GJ. Pharmacognosy [M]. 2nd ed. Beijing : People’s Health Publishing House, 1996, 389-390.
[2] Jiangsu Medical College. Dictionary of Traditional Chinese Medicine [M]. Shanghai : Shanghai Scientific and Technical Publishing House, 1995, 380-382.
[3] Sandberg F. Two glycoside-containing genera of the Araliaceae family, Panax and Eleutherococcus[J]. Planta Med, 1973, 24 (1) : 392-396.
[4] Barenboim GM, Brikenshtein VKh, Shul’man ML, et al. Luminescence analysis in pharmacological studies. III. Fluorescence of individual eleutheroside and an extract of Eleutherococcus [J]. Khim Farm Zh, 1984, 18 (4) : 493-500.
[5] Todorov IN, Sizova ST, Mitrokhin YI, et al. Study on the pharmacokinetics and mechanism of action of Eleutherococcus glycosides. VII. Effect of the extract and of individual glucosides on protein biosynthesis [J]. Khim Farm Zh, 1984, 18 (8) : 920-924.
[6] Takasugi N, Moriguchi T, Fuwa T, et al. Effect of Eleutherococcus senticosus on rectal temperature, body and grip tones, mortor coordination, and exploratory and spontaneous movement in acute stressed mice [J]. Shoyakugaku Zasshi, 1985, 39 (3) : 232-237.
[7] Solo’eva AG, Petukhova TV, Greshnykh RD. Analysis and standardization of Eleutherococcus roots and rhizomes and liquid extracts according to the content of biologically active compounds [J]. Farmatsiya, 1989, 38 (1) : 25-27.
[8] Jong SK, Pham TL, Xing FC, et al. Quantitative determination of ELU B and E from Acanthopanax species by high performance liquid chromatography [J]. Arch Pharm Res, 2001, 24 (5) : 407-411.
[9] Feng SL, Chen LR, Zhao FH, et al. Determination of eleutherosides B and Eof Acathopanax by HPLC [J]. Chin J Mod Appl Pharm, 2001, 18 (2) : 124-126.
[10] State Pharmacopoeia Committee of People’s Republic of China. Pharmacopoeia of the People’s Republic of China [S]. 2000 Ed. Beijing : Chemical Industry Press, 2000. 491.
[11] State Pharmacopoeia Committee of People’s Republic of China. Pharmacopoeia of the People’s Republic of China [S]. The Drug Standards Issned by the Ministry of Health, People’s Republic of China. 1998, Vol 18. 162-163.
[12] Wu YC, Huang SD. Solid-phase microextraction coupled with High-performance liquid chromatography for the determination of aromatic amines [J]. Anal Chem, 1999, 71 (2) : 310-318.
[13] Chen J, Pawliazyn JB. Solid phase microextraction coupled with high-performance liquid chromatography [J]. Anal Chem, 1995, 67 (5) : 2530-2533.
[14] Liu LC, Zhao FH, Chen LR. Determination of content of eleutherosides B and E in Acanthopanax by HPLC [J]. Anal Test Technol Instrum, 2000, 6 (2) : 93-94.
[15] Wang J, Liu LC. Determination of eleutherosides B, E of Acanthopanax extract by HPLC [J]. Lishizhen Medic Mat Med Res, 2003, 14 (7) : 398-399.