越婢加术汤对阿霉素肾病大鼠肾脏保护作用的药效学研究

doi:10.5246/jcps.2022.09.060

中国药学(英文版) ›› 2022, Vol. 31 ›› Issue (9): 711-717.DOI: 10.5246/jcps.2022.09.060

越婢加术汤对阿霉素肾病大鼠肾脏保护作用的药效学研究

赵峰^#, 王瑞^#^,^*(), 张洪雄, 高太祥, 王楠

山西中医药大学中药与食品工程学院, 山西晋中 030619

收稿日期:2022-05-10 修回日期:2022-06-11 接受日期:2022-06-20 出版日期:2022-09-30 发布日期:2022-09-30
通讯作者: 王瑞
作者简介:
+ Tel.: +86-351-3179903, E-mail: wrgreentea@163.com
基金资助:
Applied basic Research Project of Science and Technology Department of Shanxi Province (Grant No. 201901D-111343), the Open Project of Key Laboratory of Shanxi Province (Grant No. SXIDL-2018-09), Shanxi University of Traditional Chinese Medicine Science and Technology Innovation Ability Training program "basic Research special project" (Grant No. 2020PY-JC-20).

Pharmacodynamics of Yuebi Jiazhu Decoction on renal protection of adriamycin nephropathy rats

Feng Zhao^#, Rui Wang^#^,^*(), Hongxiong Zhang, Taixiang Gao, Nan Wang

School of Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China

Received:2022-05-10 Revised:2022-06-11 Accepted:2022-06-20 Online:2022-09-30 Published:2022-09-30
Contact: Rui Wang
About author:
# Feng Zhao and Rui Wang contributed equally to this work.

摘要/Abstract

摘要：

本实验旨在观察越婢加术汤对阿霉素肾病大鼠的肾脏保护作用。除对照组外, 其余大鼠均尾静脉注射阿霉素造模。动物模型建立成功后, 将大鼠随机分为模型组、越婢加术汤低、中、高剂量组和阳性药组。经药物干预4周后, 收集各组大鼠24 h尿样, 监测生化指标并摘取肾脏组织进行光镜病理分析。实验结果显示, 越婢加术汤各组及阳性药组大鼠尿蛋白水平均低于模型组(P < 0.05)。与模型组比较, 越婢加术汤各组及阳性药组血清BUN、SCr、TG水平均显著降低(P < 0.01), 而ALB水平显著升高(P < 0.01)。与模型组比较, 越婢加术汤各组及阳性药组大鼠肾脏组织病理损伤均有明显减轻。证明越婢加术汤对阿霉素肾病大鼠具有肾脏保护作用。

关键词: 越婢加术汤, 阿霉素肾病大鼠, 肾病综合征, 药效学

Abstract:

In the present study, we aimed to investigate the protective effect of Yuebi Jiazhu Decoction (YBJZD) on the kidney of adriamycin nephropathy (AN) rats. Rats were injected with adriamycin for modeling, except for the control group. After the successful establishment of the animal model, rats were randomly divided into the model group, YBJZD low-, medium-, and high-dose groups, and the positive group. The 24-h urine samples were collected. Biochemical indicators were monitored, and kidney tissues were collected for pathological analysis using light microscopy. The results showed that through 4 weeks of drug intervention, the urinary protein level was lower in the YBJZD and positive groups compared with the model group (P < 0.05). Serum levels of BUN, SCr, and TG were significantly lower (P < 0.01), and ALB was significantly higher (P < 0.01) in the YBJZD and positive groups compared with the model group. Compared with the model group, the pathological injury of kidney tissue in the YBJZD and positive groups was significantly alleviated. These outcomes proved that YBJZD had a renal protective effect on AN rats.

Key words: Yuebi Jiazhu Decoction, Adriamycin nephropathy rats, Nephrotic?syndrome, Pharmacodynamics

Supporting:

赵峰, 王瑞, 张洪雄, 高太祥, 王楠. 越婢加术汤对阿霉素肾病大鼠肾脏保护作用的药效学研究[J]. 中国药学(英文版), 2022, 31(9): 711-717.

Feng Zhao, Rui Wang, Hongxiong Zhang, Taixiang Gao, Nan Wang. Pharmacodynamics of Yuebi Jiazhu Decoction on renal protection of adriamycin nephropathy rats[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(9): 711-717.

图/表 4

Figure 1. Comparisons of body weights among all groups. Note: vs. control, **P < 0.01; vs. model, #P < 0.05, ##P < 0.01.

Table 1. Effect of YBJZD on quantitation of 24-h urinary protein of rats (x ± s).

Table 2. Effect of YBJZD on renal function of rats (x ± s).

Figure 2. Effect of YBJZD on renal histopathology in rats (HE, ×400). (A) Control group; (B) model group; (C) YBJZD low-dose group; (D) YBJZD medium-dose group; (E) YBJZD high-dose group; (F) positive group.

参考文献 28

[1]	Hu, F.Y.; Xie, M.Y. Effect of low molecular weight heparin calcium combined with prednisone in children with primary nephrotic syndrome. Med. Innov. China. 2019, 16, 17–20.
[2]	Li, F.X.; Hou, Y.L.; Zhou, L.S.; Dong, Y.; Zhao, J.W.; Li, Z.X. The status and model of children primary nephrotic syndrome in continuing nursing. Ann. Palliat. Med. 2021, 10, 2398–2407.
[3]	Hao, S.; Wu, Y.; Kang, Y.L.; Niu, X.; Zhu, G.; Huang, W.Y. A single-center analysis of primary nephrotic syndrome with acute pancreatitis in children. Med. (Baltimore). 2020, 27, e21056.
[4]	Cao, J.; Li, J.; Shen, H. Study on the clinical effect of benazepril combined with prednisone in treatment of pediatric patients with nephrotic syndrome. J. Clin. Exp. Med. 2019, 18, 2543–2546.
[5]	He, Y.; Zhao, X.Y. Effect of low molecular heparin combined with prednisone on the treatment of nephrotic syndrome and its influence on renal function, APTT and PT levels in children. J. North Sichuan Med. Coll. 2018, 33, 268–270.
[6]	Liu, X.R.; Mu, Y.; Liu, H.M.; Deng, C. Diagnostic value of plasma protein Z and factor VIII in primary nephrotic syndrome. Clin. study. 2019, 05, 135–136.
[7]	Yan, S.P.; Zhuang, X.L.; Ai, S.; Qiu, C.X.; Zheng, J. Discussion on the treatment of primary nephrotic syndrome from "Kai Guimen, Jiejing Fu and Quwan Chencuo". Lishizhen Med. Mater. Med. Res. 2021, 04, 931–932.
[8]	Wei, H.M.; Xiang, S.W.; Liu, W.L.; Zhong, W.L. Research Progress of Jingfang in Treating Renal Edema. Mass. Technol. 2020, 07, 83–86.
[9]	Zhang, Z.J. Synopsis of Golden Chamber. Beijing: China Medical Science Press. 2018, 45–47.
[10]	Fu, H.L. Clinical Observation of Using Yuebi Jiazhu Decoction Combined with Wuling Powder in Treating Adult Primary Nephrotic Syndrome of Wind with Water Syndrome. Sichuan Tradit. Chin. Med. 2020, 02, 125–128.
[11]	Jia, P.P.; Song, D.; Song, C.D.; Chen, Y.; Hua, S.T. The Effects of Zhenwu Decoction and Yuebi Jiazhu Decoction on the Expression of B7-1 in Renal Tissue of Model Rats with Adriamycin Nephropathy. J. Tradit. Chin. Med. 2019, 05, 989–993.
[12]	Huang, Y.F.; Wei, L.B. Ten Methods of Treating Kidney in Jin Gui Yao Lue. Chin. J. Nephrol. Integr. Tradit. West. Med. 2015, 12, 1106–1108.
[13]	Xu, K. M. S. thesis, Changchun University of Traditional Chinese Medicine. 2019.
[14]	Zhang, N.; Gao, Y.W.; LV, C.M.; Yang, X.L.; Yue, G.H. Effect of Huanglian Jiedu Decoction on vascular endothelium factor and expression of RhoA protein factor in young spontaneously hypertensive rats. J. Chin. Pharm. Sci. 2019, 28, 114–120.
[15]	Noone, D.G.; Iijima, K.; Parekh, R. Idiopathic nephrotic syndrome in children. Lancet. 2018, 392, 61–74.
[16]	Lee, V.W.; Harris, D.C. Adriamycin nephropathy: a model of focal segmental glomerulosclerosis. Nephrology. 2011, 16, 30–38.
[17]	Li, A.P.; Zhang, W.N.; Qin, X.M. Optimization of adriamycin-induced nephropathy rat model. Chin. Tradit. Herb. Drugs. 2018, 49, 151–159.
[18]	Chang, J. Effect of Guizhi Fuling Decoction combined with Prednisone Tables on Inflammatory Factors in Children with Primary Nephrotic Syndrome. Bright Tradit. Chin. Med. 2021, 13, 2236–2238.
[19]	Yu, Q. Clinical observation on the treatment of IgA nephropathy with nephrotic syndrome by integrated traditional Chinese and western medicine. China Heal. Stand. Manag. 2017, 8, 107–108.
[20]	Wen, D.F. Significance of Traditional Chinese Medicine Intervention for Primary Nephrotic Syndrome During Hormone Withdrawal Period. World Latest Med. Inf. 2019, 99, 304–305.
[21]	Tian, M.M. HUANG huang’s Clinical Experience of Yuebi Jiazhu Decoction. J. Jiangxi Univ. Tradit. Chin. Med. 2021, 06, 17–21.
[22]	Miao, S.M.; Zhang, Q.; Bi, X.B.; Cui, J.L.; Wang, M.L. A review of the phytochemistry and pharmacological activities of Ephedra herb. Chin. J. Nat. Med. 2020, 18, 321–344.
[23]	Zhao, F.; Gao, T.X.; Shi, L.Y.; Wang, R. Establishment of HPLC Fingerprints, Content Determination and Chemical Pattern Recognition Analysis of Yuebi Jiazhu Decoction. China Pharm. 2021, 32, 2724–2730.
[24]	Liu, N.; Wang, Y.Q.; Du, F.; Bian, X.H.; Sun, G.P.; Zhang, B.R.; Zhou, G.Y. Adriamycin induced a nephropathy model of focal segmental glomerulosclerosis in mice. Pract. Pharm. Clin. Remedies. 2014, 17, 1536–1540.
[25]	Soyoral, Y.; Aslan, M.; Emre, H.; Begenik, H.; Erdur, F.M.; Turkel, A.; Selek, S.; Erkoc, R. Serum paraoxonase activity and oxidative stress in patients with adult nephrotic syndrome. Atheroscler. 2011, 01, 243–246.
[26]	Tang, G.Y.; Wen, H.; Wang, J.J.; Zhang, H.J.; Liu, Y. Evaluation research of adriamycin-induced nephropathy model in rats. Drug Eval. Study. 2020, 01, 66–71.
[27]	Colucci, M.; Corpetti, G.; Emma, F.; Vivarelli, M. Immunology of idiopathic nephrotic syndrome. Pediatr. Nephrol. 2018, 33, 573–584.
[28]	Lama, G.; Luongo, I.; Tirino, G.; Borriello, A.; Carangio, C.; Salsano, M.E. T-lymphocyte populations and cytokines in childhood nephrotic syndrome. Am. J. Kidney Dis. 2002, 39, 958–965.

越婢加术汤对阿霉素肾病大鼠肾脏保护作用的药效学研究

Pharmacodynamics of Yuebi Jiazhu Decoction on renal protection of adriamycin nephropathy rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 28

相关文章 7

编辑推荐

Metrics

本文评价

[1]	刘玥华, 薛章勤, 魏剑明, 魏若梦, 殷宝栋, 刘爱芹. 奥帕膦酸钠的药物合成及活性研究[J]. 中国药学(英文版), 2022, 31(11): 883-892.
[2]	曹晶晶, 孙俊, 赵淑娟, 马培志. 流感新药巴洛沙韦的药理作用及临床评价[J]. 中国药学(英文版), 2020, 29(10): 729-733.
[3]	王芳, 陈卓, 李林, 朱明智, 熊有毅, 谷元廷. 药动学参数预测多西他赛血液毒性在中国早期乳腺癌患者中的应用[J]. 中国药学(英文版), 2016, 25(7): 512-516.
[4]	王一博, 王坚成^*, 孟萌, 张华, 张强. 多烯紫杉醇结合白蛋白纳米粒的制备及体内外评价 [J]. , 2010, 19(3): 214-222.
[5]	李馨儒, 黄燕清, 李晓燕, 周艳霞, 刘艳^*, 国明, 祝清芬, 谢元超, 范治云. 盐酸青藤碱贴剂的制备和评价[J]. , 2010, 19(2): 110-114.
[6]	张煊, 张华, 王桂玲, 张大卫, 王静, 蔺伟, 张强^*. 正常大鼠静脉注射PEG包裹胰岛素脂质体的降血糖作用[J]. , 2004, 13(1): 28-31.
[7]	董梅, 冯奉仪^*, 付强, 朱珠. 高剂量表阿霉素在肿瘤患者中的药物动力学和药效学研究[J]. , 2000, 9(2): 92-95.