酢浆草乙醇提取物的凝血止血作用和急性毒性初步探究

doi:10.5246/jcps.2022.07.046

中国药学(英文版) ›› 2022, Vol. 31 ›› Issue (7): 530-535.DOI: 10.5246/jcps.2022.07.046

酢浆草乙醇提取物的凝血止血作用和急性毒性初步探究

杨加琼¹, 刘英², 宗大庆¹, 赵静峰²^,^*()

1. 昭通卫生职业学院, 云南昭通 657000
2. 教育部自然资源药物化学重点实验室, 云南省天然产物转化与应用重点实验室,云南大学化工学院, 云南昆明 650091

收稿日期:2022-05-02 修回日期:2022-05-30 接受日期:2022-06-05 出版日期:2022-07-31 发布日期:2022-07-31
通讯作者: 赵静峰
作者简介:
+ Tel.: +86-13888063305, E-mail: 782568300@qq.com
基金资助:
Yunnan Province Education Department Scientific Research Fund Project (Grant No. 2022J1683).

Preliminary study on blood coagulation and hemostatic effect and acute toxicity of Oxalis corniculata L. ethanol extract

Jiaqiong Yang¹, Ying Liu², Daqing Zong¹, Jingfeng Zhao²^,^*()

1 Zhaotong Health Vocational College, Zhaotong 657000, Yunnan, China
2 Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education; Yunnan Research & Development Center for Natural Products; School of Chemical Science and Technology, University, Kunming 650091, Yunnan, China

Received:2022-05-02 Revised:2022-05-30 Accepted:2022-06-05 Online:2022-07-31 Published:2022-07-31
Contact: Jingfeng Zhao

摘要/Abstract

摘要：

本文主要观察酢浆草乙醇提取物对小鼠出血时间(BT)、凝血时间(CT)和急性毒性作用。首先取40只小鼠, 随机均分为正常对照组、云南白药组、酢浆草乙醇提取物低剂量组和高剂量组。给药组以酢浆草乙醇提取物低剂量(0.6 g/kg)、高剂量(1.2 g/kg)灌胃, 应用玻片法、剪尾法测定小鼠的凝血时间(CT)、止血时间(BT); 再取50只小鼠, 采用半数致死量法, 对小鼠进行灌胃给药, 观察小鼠生理状态、中毒反应及死亡, 于试验第14天剖检观察其脏器有无异常情况。结果在凝血止血试验中, 我们发现各组间无统计学差异(P > 0.05), 但具有明显的剂量反应关系, 且BT时间和CT时间明显低于阴性对照组, 和云南白药对照组相当。在急性毒性试验中, 酢浆草乙醇提取物半数致死量为6.0291 g/kg, 95%可信区间为5.3065–6.7829 mg/kg。

关键词: 酢浆草乙醇提取物, 止血作用, 凝血时间, 急性毒性

Abstract:

In the present study, we aimed to observe the effects of ethanol extract of Oxalis corniculata L. on bleeding time (BT) and coagulation time (CT) and determine its acute toxicity in mice. Firstly, the ethanol extract of O. corniculata was administered to 40 mice, which were randomly divided into the normal control group, Yunnan Baiyao control group, low-dose group, and high-dose group. The low-dose (0.6 g/kg) and high-dose (1.2 g/kg) groups received ethanol extract of O. corniculata by gavage, and the BT and CT of the mice were measured by the slide method and tail clipping method, respectively. Secondly, the median lethal dose method was used with 50 mice to observe the physiological state, poisoning reaction, and death of the mice after intragastric administration. Finally, on the 14th day of the experiment, a necropsy was performed to observe any abnormality of the organs. In conclusion, in the coagulation and hemostasis tests, there were no statistical differences between the groups (P > 0.05), while there was a significant dose-response relationship, and the BT and CT were significantly shorter than those of the negative control group, which were comparable to the Yunnan Baiyao control group. Moreover, in the acute toxicity test, the median lethal dose of ethanol extract of O. corniculata was 6.0291 g/kg, with a 95% confidence interval of 5.3065–6.7829 mg/kg.

Key words: Ethanol extract of Oxalis corniculata L., Hemostatic effect, Coagulation time, Acute toxicity

Supporting:

杨加琼, 刘英, 宗大庆, 赵静峰. 酢浆草乙醇提取物的凝血止血作用和急性毒性初步探究[J]. 中国药学(英文版), 2022, 31(7): 530-535.

Jiaqiong Yang, Ying Liu, Daqing Zong, Jingfeng Zhao. Preliminary study on blood coagulation and hemostatic effect and acute toxicity of Oxalis corniculata L. ethanol extract[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(7): 530-535.

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酢浆草乙醇提取物的凝血止血作用和急性毒性初步探究

Preliminary study on blood coagulation and hemostatic effect and acute toxicity of Oxalis corniculata L. ethanol extract

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