β-葡聚糖苷酶对三七皂苷-Fe的转化研究

β-葡聚糖苷酶对三七皂苷-Fe的转化研究

姜彬慧, 赵余庆^*, 韩颖, 崔宇, 胡筱敏

1.东北大学资源与土木工程学院, 辽宁沈阳 110004;
2.辽宁中医学院, 辽宁沈阳 110032

收稿日期:2004-10-25 修回日期:2006-02-10 出版日期:2006-03-15 发布日期:2006-03-15
通讯作者: 赵余庆*

Enzymatic Transformation of Notoginsenoside Fe by β-Glucanase

JIANG Bin-hui, ZHAO Yu-qing^*, HAN Ying, CUI Yu, HU Xiao-min

1.College of Resources and Civil Engineering, Northeast University, Shenyang 110004, China;
2.Liaoning College of Traditional Chinese Medicine, Shenyang 110032,China

Received:2004-10-25 Revised:2006-02-10 Online:2006-03-15 Published:2006-03-15
Contact: ZHAO Yu-qing*

摘要/Abstract

摘要： 目的本文首次报道利用工业酶制剂对三七皂苷-Fe进行酶转化的研究。方法从三七茎叶总皂苷中分离鉴定三七皂苷-Fe, 利用工业酶制剂β-葡聚糖苷酶对三七皂苷-Fe进行转化。树脂法、硅胶柱层析及薄层层析法分离、纯化酶转化产物; ¹H NMR和¹³C NMR光谱数据分析鉴定酶转化产物Ⅰ是20(S)原人参二醇-20-O-α-L-呋喃阿拉伯糖基(1→6)β-D-吡喃葡萄糖苷(人参皂苷-Mc); 转化物Ⅱ是20(S)原人参二醇-20-O-β-D-吡喃葡萄糖苷(C-K)。结果酶解动力学的研究中, 经过24h的反应, 三七皂苷-Fe的转化率接近95%。结论 β-葡聚糖苷酶转化三七皂苷-Fe的途径为三七皂苷-Fe→人参皂苷-Mc→C-K。,

关键词: 酶转化, 酶转化, 酶转化, 三七皂苷-Fe, 三七皂苷-Fe, 三七皂苷-Fe, β-葡聚糖苷酶, β-葡聚糖苷酶, β-葡聚糖苷酶, 工业酶制剂, 工业酶制剂, 工业酶制剂

Abstract: Aim An industrial enzyme β-glucanase was used to transform notoginsenoside Fe for the first time. Methods Notoginsenoside Fe was isolated from the leave saponin of Panax notoginseng (Burk.) Chen FH. The enzymatically transformed compounds were detected by HPLC and two transformed compounds were identified as 20(S)-protopanaxadiol-20-O-α-L-arabinofuranosyl(1→6)-β-glucopyranoside, ginsenoside-Mc) and 20(S)-protopanaxadiol-20-O-β-D-glucopyranoside compound-K (C-K) respectively on the basis of their ¹H NMR and ¹³C NMR spectral data. Results Based on the enzymolytic kinetic curve, the transformation rate of notoginsenoside Fe reached 95% after 24 h. Conclusion The enzymatic transformation pathway of notoginsenoside Fe by β-glucanase has been proposed as notoginsenoside Fe→ginsenoside Mc→C-K.

Key words: enzymatic transformation, enzymatic transformation, notoginsenoside Fe, notoginsenoside Fe, β-glucanase, β-glucanase, industrial enzyme, industrial enzyme

中图分类号:

R284.1

R915.3

Supporting: Foundation item: Ministry of Education Scientific Research Starting Foundation of Doctors Returned from Abroad (No.[2001]498); Modernization of Chinese Traditional Medicine Technology Center (No.2002403004).
^*Corresponding author. Tel.: 86-24-86224725

姜彬慧, 赵余庆^*, 韩颖, 崔宇, 胡筱敏. β-葡聚糖苷酶对三七皂苷-Fe的转化研究[J]. .

JIANG Bin-hui, ZHAO Yu-qing^*, HAN Ying, CUI Yu, HU Xiao-min. Enzymatic Transformation of Notoginsenoside Fe by β-Glucanase[J]. .

参考文献

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