DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究

doi:10.5246/jcps.2017.05.037

中国药学(英文版) ›› 2017, Vol. 26 ›› Issue (5): 355-359.DOI: 10.5246/jcps.2017.05.037

DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究

杨登峰¹, 刘凤楼³, 杨小龙^2*

1. 广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心, 广西南宁 530007
2. 重庆大学药学院创新药物研究中心, 重庆 401331
3. 宁夏大学农学院, 宁夏银川 750021

收稿日期:2017-03-09 修回日期:2017-03-23 出版日期:2017-05-26 发布日期:2017-05-09
通讯作者: Tel.: +86-023-65678450, E-mail: yxl19830915@163.com
基金资助:
National Natural Science Foundation (Grant No. 31660251), Fundamental Research Funds for the Central Universities (Grant No. 0903005203401), and the Start-up Fund for the “Hundred Young-Talent Scheme” Professorship (Grant No. 0236011104424).

DNA methyltransferase inhibitor dramatically alters the secondary metabolism of Pestalotiopsis microspora

Dengfeng Yang¹, Fenglou Liu³, Xiaolong Yang^2*

1. State Key Laboratory of Enzyme Technology, National Engineering Research Centre of Non-food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China
2. Innovative Drug Research Centre (IDRC), School of Pharmaceutical Sciences, Chongqing University (Huxi Campus), Chongqing 401331, China
3. Agricultural College, Ningxia Univerisity, Yinchuan 750021, China

Received:2017-03-09 Revised:2017-03-23 Online:2017-05-26 Published:2017-05-09
Contact: Tel.: +86-023-65678450, E-mail: yxl19830915@163.com
Supported by:
National Natural Science Foundation (Grant No. 31660251), Fundamental Research Funds for the Central Universities (Grant No. 0903005203401), and the Start-up Fund for the “Hundred Young-Talent Scheme” Professorship (Grant No. 0236011104424).

摘要/Abstract

摘要：

DNA甲基转移酶抑制剂5氮杂胞苷导致了植物内生真菌小孢拟盘多毛孢次级代谢产物的明显变化。分析发酵液萃取物分离到一个新化合物4′-formamidophenyl-5-methoxybenzoate (1), 和七个已知的聚酮4-hydroxybenzoic acid (2), LL-P880α (3), 1′-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4), pestalotiollide B (5), pestalotiopyrone G (6), endocrocin (7), and 2′-hydroxy-6′-hydroxymethyl-4′-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8)。液相分析结果显示除了4以外,其他所有化合物都是新诱导产生的。所有化合物被证明没有明显的抗真菌活性。

关键词: 遗传表观修饰, 植物内生真菌, 小孢拟盘多毛孢, 聚酮, 抗真菌活性

Abstract:

5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4′-formamidophenyl-5-methoxybenzoate (1), along with seven known polyketides, 4-hydroxybenzoic acid (2), LL-P880α (3), 1′-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4), pestalotiollide B (5), pestalotiopyrone G (6), endocrocin (7) and 2′-hydroxy-6′-hydroxymethyl-4′-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8). HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.

Key words: Epigenetic modifier, Plant endophytic fungi, Pestalotiopsis microspora, Polyketide, Antifungal activity

中图分类号:

R284

Supporting:

杨登峰, 刘凤楼, 杨小龙. DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究[J]. 中国药学(英文版), 2017, 26(5): 355-359.

Dengfeng Yang, Fenglou Liu, Xiaolong Yang. DNA methyltransferase inhibitor dramatically alters the secondary metabolism of Pestalotiopsis microspora[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(5): 355-359.

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DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究

DNA methyltransferase inhibitor dramatically alters the secondary metabolism of Pestalotiopsis microspora

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