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中国药学(英文版) ›› 2016, Vol. 25 ›› Issue (3): 224-227.DOI: 10.5246/jcps.2016.03.027

• 【简报】 • 上一篇    下一篇

翻白草三萜类成分及其PTP1B抑制活性研究

脱振东, 李娜, 李佳琳, 齐世洲, 李班班, 张乐, 崔龙*   

  1. 北华大学 药学院, 吉林省 吉林市 132013
  • 收稿日期:2015-10-25 修回日期:2015-11-28 出版日期:2016-03-29 发布日期:2015-12-09
  • 通讯作者: Tel.: +86-13604471092, +86-18604498621, E-mail: cuilong71@163.com
  • 基金资助:
    Science and Technology Development Program of Jilin Province (Grant No. 20150101225JC).

Inhibition of protein tyrosine phosphatase 1B by triterpenes isolated from Potentilla discolor Bge

Zhendong Tuo, Na Li, Jialin Li, Shizhou Qi, Banban Li, Le Zhang, Long Cui*   

  1. College of Pharmacy, Beihua University, Jilin 132013, China
  • Received:2015-10-25 Revised:2015-11-28 Online:2016-03-29 Published:2015-12-09
  • Contact: Tel.: +86-13604471092, +86-18604498621, E-mail: cuilong71@163.com
  • Supported by:
    Science and Technology Development Program of Jilin Province (Grant No. 20150101225JC).

摘要:

对翻白草的化学成分进行研究, 从中分离得到7个三萜类化合物, 分别鉴定为3-oxoolean-12-en-27-oic acid (1),gypsogenic acid (2),3α-hydroxyolean-12-en-27-oic acid (3), 3β-hydroxyolean-12-en-27-oic acid (4),aceriphyllic acid A (5), aceriphyllic acid A methyl ester (6)oleanolic acid (7)。对化合物17进行了抑制PTP1B活性的实验, IC50范围为(7.5±0.5)(22.7±0.5) μmol/L。其中, 化合物1, 2, 37表现出对PTP1B的选择抑制性。

关键词: 翻白草, 蛋白酪氨酸磷酸酯酶1B, 三萜类化合物

Abstract:

Seven oleanene triterpenes were isolated from the roots of Potentilla discolor Bge and their structures were identified as3-oxoolean-12-en-27-oic acid (1), gypsogenic acid (2), 3α-hydroxyolean-12-en-27-oic acid (3), 3β-hydroxyolean-12-en-27-oic acid (4), aceriphyllic acid A (5), aceriphyllic acid A methyl ester (6), and oleanolic acid (7). Compounds 17 inhibited protein tyrosine phosphatase 1B (PTP1B) activity, with IC50 values ranging from (7.5±0.5) to (22.7±0.5) μmol/L. Among the isolates, compounds 1, 2, 3 and 7 from thePotentilla discolor Bge were found to exhibit selective PTP1B inhibitory activity.

Key words: Potentilla discolor Bge, Protein tyrosine phosphatase 1B, Triterpenes

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