金银花与其四倍体九丰一号化学成分的比较分析

doi:10.5246/jcps.2015.12.103

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (12): 809-815.DOI: 10.5246/jcps.2015.12.103

金银花与其四倍体九丰一号化学成分的比较分析

张潇¹, 郭青^2*, 余伯阳^1*, 李敏³, 霍丽霞¹, 王俊俊¹

1. 中国药科大学中药学院中药复方教研室, 江苏南京 211198
2. 江苏省食品药品监督检验研究院, 江苏南京 210008
3. 南京中医药大学, 江苏南京 210009

收稿日期:2015-08-27 修回日期:2015-10-01 出版日期:2015-12-22 发布日期:2015-10-11
通讯作者: Tel.: 025-86632807, E-mail: jssjik@163.com, guoqing850@sohu.com

Qualitative analysis of the chemical constituents of Lonicera japonica and its tetraploid cultivar Jiufengyihao

Xiao Zhang¹, Qing Guo^2*, BoyangYu^1*, Ming Li³, Lixia Huo¹, Junjun Wang¹

1. Department of Complex Prescription of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing, 211198, China
2. Jiangsu Institute for Food and Drug Control, Nanjing, 210008, China
3. Nanjing University of Traditional Chinese Medicine, Nanjing 210009, China

Received:2015-08-27 Revised:2015-10-01 Online:2015-12-22 Published:2015-10-11
Contact: Tel.: 025-86632807, E-mail: jssjik@163.com, guoqing850@sohu.com

摘要/Abstract

摘要：

本文首次采用UPLC-IT-TOF/MS方法对金银花和它的四倍体九丰一号的化学成分进行了比较分析。采用C₁₈超微色谱柱(100 mm×2.1 mm, 1.8 μm)进行分离, 乙腈-0.1%甲酸为流动相梯度洗脱。根据精确分子量、特征性MS²离子碎片和各类化合物的裂解规律, 结合对照品和参考文献, 从金银花和九丰一号中共鉴定了30个化合物, 其中11个酚酸类, 10个环烯醚萜类, 8个黄酮类和1个皂苷类。比较分析结果表明, 金银花含有全部30个化合物, 而九丰一号只含有21个; 此外二者各共有峰所占的相对比例也不一样, 说明二者化学成分存在着较大差异。

关键词: UPLC-IT-TOF/MS, 金银花, 九丰一号, 四倍体

Abstract:

In the present study, we, for the first time, developed an UPLC-IT-TOF/MS method to compare the chemical constituentsof the flower buds of Lonicera japonicaThunb. (Jinyinhua, JYH, in Chinese) and its tetraploid cultivar Jiufengyihao (JFYH). The samples were separated on a C₁₈ ultra-column (100 mm×2.1 mm, 1.8 μm) eluted with a mixture of acetonitrile and 0.1% formic acid using a gradient program. Based on the accurate masses, fragmental MS² ions and fragmentation pathway, we unambiguously affirmed or tentatively identified 30 compounds, including 11 phenolic acids, 10 iridoid glycosides, 8 flavonoids and 1 saponin, from JYH and JFYH. Among them, all compounds were detected in Lonicera japonica, but only 21 compounds were detected in JFYH. In addition, the relative contents of the 21 common chemical constituents were also different in JYH and JFYH, suggesting an obvious chemical difference between JYH and JFYH.

Key words: UPLC-IT-TOF/MS, Lonicera japonica, Jiufengyihao, Tetraploid cultivar

中图分类号:

R284

Supporting:

张潇, 郭青, 余伯阳, 李敏, 霍丽霞, 王俊俊. 金银花与其四倍体九丰一号化学成分的比较分析[J]. 中国药学(英文版), 2015, 24(12): 809-815.

Xiao Zhang, Qing Guo, BoyangYu, Ming Li, Lixia Huo, Junjun Wang. Qualitative analysis of the chemical constituents of Lonicera japonica and its tetraploid cultivar Jiufengyihao[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(12): 809-815.

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金银花与其四倍体九丰一号化学成分的比较分析

Qualitative analysis of the chemical constituents of Lonicera japonica and its tetraploid cultivar Jiufengyihao

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