不同的多糖手性固定相对萘哌地尔及其衍生物的对映体进行HPLC光学异构体拆分的比较

不同的多糖手性固定相对萘哌地尔及其衍生物的对映体进行HPLC光学异构体拆分的比较

刘夏雯, 袁牧^*, 季红

广州医学院药物研究中心, 广东广州 510182

收稿日期:2010-03-22 修回日期:2010-06-10 出版日期:2010-07-15 发布日期:2010-07-15
通讯作者: 袁牧*

Comparison of different polysaccharide-based chiral stationary phases for enantioselective resolution of Naftopidil and its derivatives by HPLC

Xia-Wen Liu, Mu Yuan^*, Hong Ji

The Pharmaceutical Research Center of Guangzhou Medical College, Guangzhou 510182, China

Received:2010-03-22 Revised:2010-06-10 Online:2010-07-15 Published:2010-07-15
Contact: Mu Yuan*

摘要/Abstract

摘要： 完成了以几种多糖衍生物为手性固定相, 拆分Naftopidil及其衍生物光学异构体的高效液相色谱方法。正相模式下, 运用的手性固定相包括四种涂敷型以及两种键和型手性色谱柱,流动相为正己烷、异丙醇和二乙胺一定比例混合液。多糖三 (3,5-二甲苯基氨基甲酸酯) 被证实为分离该类化合物最佳的手性选择剂。在极性条件以及反相条件下拆分该类化合物光学异构体的研究由键和型手性柱Chiralpak IA 和Chiralcel IB 完成。两者在纯甲醇和含水条件下对题中大多数化合物均具有良好的对映体选择性。另外,本文还讨论了三种模式下的各个手性固定相对分析物可能的手性识别机理。

关键词: 多糖手性固定相, 对映体选择性拆分, 萘哌地尔及其衍生物

Abstract:

Effective enantioseparation of Naftopidil and its derivatives by HPLC was accomplished using several different polysaccharide-based chiral stationary phases (CSPs). In normal-phase mode, the compounds were eluted on four coated- and two immobilized-columns with the mixture of n-hexane, isopropanol and diethylamine (DEA). Polysaccharide tris(3,5-dimethylphenyl carbamate) was shown to be the best enantiomer selector. In addition, the immobilized column packed with Chiralpak IA or IB was applied under polar-organic and reversed-phase conditions, both of which exhibited excellent enantioselectivity for Naftopidil and its derivatives. Furthermore, the underlying possible chiral recognition mechanisms were discussed.

Key words: Polysaccharide-based chiral stationary phases, Enantioselective separation, Naftopidil and its derivatives

中图分类号:

R917.01

Supporting: Foundation item: Guangzhou mega Projects of Science Research in 2009 (Grant No. 2009A1-E011-7). ^*Corresponding author. Tel.: 86-20-81340727

刘夏雯, 袁牧^*, 季红. 不同的多糖手性固定相对萘哌地尔及其衍生物的对映体进行HPLC光学异构体拆分的比较[J]. .

Xia-Wen Liu, Mu Yuan^*, Hong Ji . Comparison of different polysaccharide-based chiral stationary phases for enantioselective resolution of Naftopidil and its derivatives by HPLC [J]. .