高效液相色谱法同时测定红花药材及红花注射液中4种核苷的含量

高效液相色谱法同时测定红花药材及红花注射液中4种核苷的含量

周斐然, 赵明波, 屠鹏飞^*

北京大学天然药物及仿生药物国家重点实验室; 药学院天然药物学系, 北京 100191

收稿日期:2009-05-29 修回日期:2009-10-15 出版日期:2009-11-30 发布日期:2009-11-30
通讯作者: 屠鹏飞*

Simultaneous determination of four nucleosides in Carthamus tinctorius L.
and Safflower injection using high-performance liquid chromatography

Fei-Ran Zhou, Ming-Bo Zhao, Peng-Fei Tu^*

State Key Laboratory of Natural and Biomimetic Drugs; Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China

Received:2009-05-29 Revised:2009-10-15 Online:2009-11-30 Published:2009-11-30
Contact: Peng-Fei Tu*

摘要/Abstract

摘要：

测定红花药材及红花注射液中4种核苷类成分胞苷、尿苷、鸟苷和腺苷含量。采用 Zorbax Eclipse XDB-C18色谱柱; 以0.05% 三氟乙酸水溶液(A)和甲醇(B)为流动相梯度洗脱, 在25 ºC 条件下流速为1 mL/min; 检测波长为260 nm。胞苷、尿苷、鸟苷、腺苷和尿嘧啶线性范围分别为4.02-603 μg/mL (r² = 0.9998), 9.38-1407 μg/mL (r² = 0.9999), 80.6-8060 μg/mL (r² = 0.9999) 及2.10-630 μg/mL (r² = 0.9987); 加样回收率分别为97.2%, 94.5%, 98.6%及108.5%。红花药材及红花注射液中4种核苷类成分的含量差异显著。本文首次报道了红花药材及红花注射液中核苷类成分的含量, 其对完善红花药材及红花注射液的质量控制具有一定的指导意义。

关键词: 红花, 红花注射液, 核苷, 高效液相色谱法

Abstract: We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid (TFA) aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL/min at 25 °C with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of 4.02-603 μg/mL (r² = 0.9998), 9.38-1407 μg/mL (r² = 0.9999), 80.6-8060 μg/mL (r² = 0.9999) and 2.10-630 μg/mL (r²= 0.9987) with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.

Key words: Carthamus tinctorius L., Carthamus tinctorius L., Safflower injection, Safflower injection, Nucleoside, Nucleoside, High-performance liquid chromatography, High-performance liquid chromatography

中图分类号:

R931.5

Supporting:

Foundation item: Program for Changjiang Scholar and Innovative Team in University (Grant No. 985-2-063-112).
^*Corresponding author. Tel./fax: 86-10-82802750; e-mail: pengfeitu@vip.163.com

周斐然, 赵明波, 屠鹏飞^*. 高效液相色谱法同时测定红花药材及红花注射液中4种核苷的含量[J]. .

Fei-Ran Zhou, Ming-Bo Zhao, Peng-Fei Tu^*. Simultaneous determination of four nucleosides in Carthamus tinctorius L.
and Safflower injection using high-performance liquid chromatography [J]. .

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