半夏生物总碱对癫痫大鼠海马氨基酸含量和GABAA受体mRNA表达的影响

半夏生物总碱对癫痫大鼠海马氨基酸含量和GABA_A受体mRNA表达的影响

古艳婷, 马永刚, 王明正^*, 王新风, 肖奕

山西医科大学药理教研室, 山西太原 030001

收稿日期:2009-04-14 修回日期:2009-08-10 出版日期:2009-09-15 发布日期:2009-09-15
通讯作者: 王明正*

The effects of pinellia total alkaloids on the amino acid concentration and the GABA_A receptor expression in hippocampus region of epileptic rats

Yan-Ting Gu, Yong-Gang Ma, Ming-Zheng Wang^*, Xin-Feng Wang, Yi Xiao

Department of Pharmacology, Shanxi Medical University, Taiyuan 030001, Shanxi, China

Received:2009-04-14 Revised:2009-08-10 Online:2009-09-15 Published:2009-09-15
Contact: Ming-Zheng Wang*

摘要/Abstract

摘要：

研究半夏生物总碱(PTA)对青霉素慢性点燃癫痫模型大鼠的对抗作用, 以及对海马区谷氨酸、天冬氨酸、甘氨酸、GABA和GABAA受体mRNA表达的影响, 并探讨其抗癫痫机制。通过青霉素慢性点燃癫痫模型大鼠观察PTA对癫痫潜伏期、发作程度的影响; 采用高效液相色谱法测定海马区谷氨酸、天冬氨酸、甘氨酸、GABA的含量。同时用逆转录PCR技术来分析PTA对大脑GABAA 受体mRNA表达的影响。与正常对照组相比, 模型对照组大鼠GABA和甘氨酸水平减少, 谷氨酸和天冬氨酸水平增加, GABAA 受体mRNA表达减少; 而高、低剂量PTA均可以延长青霉素诱发癫痫的潜伏期, 并能减轻其发作程度, 同时可显著增加GABA以及GABAA 受体mRNA水平, 减少谷氨酸的水平, 而对甘氨酸和天冬氨酸无明显影响。总之, PTA对大鼠青霉素慢性点燃模型有对抗作用, 它可以增加γ-氨基丁酸含量和GABAA 受体mRNA水平, 以及降低谷氨酸含量。

关键词: 半夏生物总碱, 青霉素慢性点燃, 抗癫痫作用, 神经递质, GABAA受体mRNA表达

Abstract:

The antiepileptic effect of pinellia total alkaloids (PTA) on penicillin (PNC) chronically kindled rats was investigated. We investigated the effects of PTA on Glu, Asp, Gly and γ-aminobutyric acid (GABA) concentrations and the expression level of cerebral GABAA receptor in hippocampus. The influence of PTA on epilepsy seizure latency and degree in PNC chronically kindled rats were observed. High performance liquid chromatography (HPLC) was adopted to measure the concentrations of Glu, Asp, Gly and GABA in hippocampus. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine the expression of cerebral GABAA receptor mRNA. Compared with normal rats, the levels of GABA and Gly decreased obviously while the level of Glu and Asp increased significantly in model rats. The cerebral GABAA receptor mRNA level was also decreased at the same time. The difference was statistically different compared to the control group. PTA could prolong the latent period of the penicillin induced seizure and weaken the extent of seizure, compared with the model group without PTA treatment. Moreover, PTA increased the level of GABA and the expression level of GABAA receptor, while decreased the level of Glu significantly. However, it had no obvious effect on the level of Gly and Asp. Pre-treatment of PTA can also increase the GABAA receptor mRNA level. In conclusion, PTA could alleviate the PNC chronically kindled rat seizure. It increased the GABA level and the expression of GABAA receptor, and it decreased the Glu concentration.

Key words: Pinellia total alkaloids PTA, Pinellia total alkaloids PTA, PNC chronically kindled rats, PNC chronically kindled rats, Anticonvulsant action, Anticonvulsant action, Neurotransmitters, Neurotransmitters, GABAA receptor, GABAA receptor

中图分类号:

R965.1

Supporting:

Foundation item: Natural Science Foundation of Shanxi Province (Grant No. 20041109).
^*Corresponding author. Tel.: 13934649769; e-mail: wang5695695@163.com

古艳婷, 马永刚, 王明正^*, 王新风, 肖奕. 半夏生物总碱对癫痫大鼠海马氨基酸含量和GABA_A受体mRNA表达的影响[J]. .

Yan-Ting Gu, Yong-Gang Ma, Ming-Zheng Wang^*, Xin-Feng Wang, Yi Xiao . The effects of pinellia total alkaloids on the amino acid concentration and the GABA_A receptor expression in hippocampus region of epileptic rats [J]. .

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