不同痘病毒属病毒抗原剂量对实验鸡免疫效果的比较性研究

不同痘病毒属病毒抗原剂量对实验鸡免疫效果的比较性研究

张小莺^*, Rüdiger Schade, Heinz Ellerbrok

1. 德国柏林洪堡大学夏洛特医学院药理研究所;
2. 德国卫生部罗伯特-科赫研究所

收稿日期:2008-09-20 修回日期:2008-12-05 出版日期:2008-12-15 发布日期:2008-12-15
通讯作者: 张小莺

Apparent dose dependent antibody response in chickens immunized with orthopox virus antigens

Xiao-Ying Zhang^*, Rüdiger Schade, Heinz Ellerbrok

1. Institut für Pharmakologie, Universitätsklinikum Charité Berlin, Dorotheenstr 94, 10117 Berlin, Germany;
2. Centre for Biological Safety, Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany

Received:2008-09-20 Revised:2008-12-05 Online:2008-12-15 Published:2008-12-15
Contact: Xiao-Ying Zhang

摘要/Abstract

摘要：

本研究以两种灭活痘病毒属病毒(vaccinia virus, 7.0 × 106 PFU 和cowpox virus, 9.2 × 107 PFU)为免疫原, 分别考察不同剂量下(抗原浓度相差约100倍)实验鸡的免疫效果和相应卵黄抗体效价(免疫荧光法和抗体中和效价)。我们的研究结果, 结合文献研究显示, 抗原剂量对于特异性卵黄抗体的产生和表达水平具有重要影响。提高免疫原的浓度通常有助于提高抗体效价。但由于不同抗原的免疫原性差异, 也有可能导致免疫抑制。针对不同免疫原似乎存在最佳免疫剂量范围。

关键词: 卵黄抗体(IgY), 卵黄抗体(IgY), 卵黄抗体(IgY), 痘病毒属病毒(OPV), 痘病毒属病毒(OPV), 痘病毒属病毒(OPV), 抗原剂量, 抗原剂量, 抗原剂量, 免疫反应, 免疫反应, 免疫反应, , ,

Abstract:

Dose-dependent IgY antibody response to different amounts of orthopox virus (OPV) antigen has been studied in immunized chickens for two different OPV strains (vaccinia virus, 7.0×106 PFU and cowpox virus, 9.2×107PFU). The antibody responses to different immunizations were tested and compared by indirect immunofluorescence antibody test. Our results, together with the literature, show that the antigen dose used for immunization plays an important role for the production of specific Abs. An increase in antigen concentration may achieve higher Ab titers but, dependent on the immunogenicity of OPV antigen, it can also lead to an immune depression. However, in this study we found that OPV played a positive correlation between antigen concentration and Ab-titer.

Key words: IgY, IgY, Orthopox virus, Orthopox virus, Antigen dose, Antigen dose, Immune response, Immune response, ,

Supporting: ^*Corresponding author. Tel.: +49 30 450 525 020

张小莺^*, Rüdiger Schade, Heinz Ellerbrok
. 不同痘病毒属病毒抗原剂量对实验鸡免疫效果的比较性研究
[J]. .

Xiao-Ying Zhang^*, Rüdiger Schade, Heinz Ellerbrok
. Apparent dose dependent antibody response in chickens immunized with orthopox virus antigens
[J]. .