关键词: 当归龙荟片, 当归龙荟片, 当归龙荟片, 芦荟苷, 芦荟苷, 芦荟苷, 黄芩素, 黄芩素, 黄芩素, 芦荟大黄素, 芦荟大黄素, 芦荟大黄素, 汉黄芩素, 汉黄芩素, 汉黄芩素, 高效液相色谱法, 高效液相色谱法, 高效液相色谱法, , ,

Abstract:

high-performance liquid chromatography (HPLC) method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloin, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compounds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid (220: 4: 200, v/v/v) and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 μg/mL (r = 0.9992) for aloin, 0.4050 - 8.100 μg/mL (r = 0.9994) for baicalein, 0.1100 - 2.200 μg/mL (r = 0.9997) for aloe-emodin, 0.2160 - 4.320 μg/mL (r = 0.9991) for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% (RSD = 0.88%), 101.0% (RSD = 0.89%), 100.0% (RSD = 1.3%) and 100.1% (RSD = 1.1%) for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.

Key words: Dangguilonghui tablet, Dangguilonghui tablet, Aloin, Aloin, Baicalein, Baicalein, Aloe-emodin, Aloe-emodin, Wogonin, Wogonin, HPLC, HPLC, ,