液相微萃取/后萃取—高效液相色谱法测定厚朴及其制剂中厚朴酚与和厚朴酚的含量

液相微萃取/后萃取—高效液相色谱法测定厚朴及其制剂中厚朴酚与和厚朴酚的含量

王晓园, 陈璇, 全红, 白小红*

山西医科大学药学院, 山西太原 030001

收稿日期:2007-12-20 修回日期:2008-05-10 出版日期:2008-06-15 发布日期:2008-06-15
通讯作者: 白小红*

Determination of magnolol and honokiol in traditional Chinese medicine
Magnolia officinalis and its preparations by liquid-phase microextractionback
extraction combined with high performance liquid chromatography

Xiao-Yuan Wang, Xuan Chen, Hong Quan, Xiao-Hong Bai*

College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China

Received:2007-12-20 Revised:2008-05-10 Online:2008-06-15 Published:2008-06-15
Contact: Xiao-Hong Bai*

摘要/Abstract

摘要：

本文采用液相微萃取/后萃取 (LPME/BE) 与高效液相色谱联用法萃取并测定了中药厚朴及其制剂—藿香正气口服液和香砂养胃丸中厚朴酚与和厚朴酚的含量。优化了萃取效率影响因素, 萃取溶剂、供相与接受相、转速及萃取时间。厚朴酚与和厚朴酚的线性范围分别为1.56 - 156 μg/mL和1.10 - 110 μg/mL; 检测限 (S/N = 3) 分别为0.10 μg/mL和0.07 μg/mL, 回收率范围为98.3% - 105.1%, RSD < 2.5%。

关键词: 液相微萃取/后萃取, 液相微萃取/后萃取, 液相微萃取/后萃取, 厚朴酚, 厚朴酚, 厚朴酚, 和厚朴酚, 和厚朴酚, 和厚朴酚, 高效液相色谱法, 高效液相色谱法, 高效液相色谱法, , ,

Abstract:

Liquid-phase microextraction with back extraction (LPME-BE) combined with high performance liquid chromatography (HPLC) was investigated for the extraction and determination of magnolol and honokiol in Magnolia officinalis, a traditional Chinese medicine (TCM), and its pharmaceutical preparations, Huo Xiang Zheng Qi peroral liquid and Xiang Sha Yang Wei pellet. Organic solvent, donor and acceptor phases, stirring rate and extraction times were all factors which can influence the efficiency of extraction and were all optimized during the course of this work. Linear calibration curves were obtained in concentration ranges of 1.56-156 μg/mL for magnolol and 1.10-110 μg/mL for honokiol. Detection limits (S/N = 3) were 0.10 and 0.07 μg/mL, respectively. The relative recoveries were both in the range of 98.3% - 105.1% and RSD was lower than 2.5%.

Key words: LPME-BE, LPME-BE, Magnolol, Magnolol, Honokiol, Honokiol, HPLC, HPLC, ,

中图分类号:

R927.2

Supporting:

王晓园, 陈璇, 全红, 白小红*.

液相微萃取/后萃取—高效液相色谱法测定厚朴及其制剂中厚朴酚与和厚朴酚的含量

[J]. .

Xiao-Yuan Wang, Xuan Chen, Hong Quan, Xiao-Hong Bai*. Determination of magnolol and honokiol in traditional Chinese medicine
Magnolia officinalis and its preparations by liquid-phase microextractionback
extraction combined with high performance liquid chromatography[J]. .

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