关键词: 淫羊藿苷, 淫羊藿苷, 淫羊藿苷, 血管紧张素 II, 血管紧张素 II, 血管紧张素 II, 人脐静脉内皮细胞株(ECV-304), 人脐静脉内皮细胞株(ECV-304), 人脐静脉内皮细胞株(ECV-304), 一氧化氮, 一氧化氮, 一氧化氮

Abstract:

To investigate the effects of icariin (ICA) on angiotensin II(Ang II)-induced injury in human umbilical vein endothelial cells line (ECV-304). The ECV-304 cells were cultured in vitro. After 24 h incubating with icariin, the model of AngII-induced injury in ECV-304 was established. The cell viability (MTT method), Lactate dehydrogenase (LDH) release and Nitric oxide (NO) production in the medium, the capacity of scavenging superoxide anion radicals (O₂^·–) and hydroxyl radicals (·OH) were measured. The activities of superoxide dismutase (SOD), total nitric oxide synthase (T-NOS), inducible nitric oxide synthase (iNOS) and constitutive nitric oxide synthase (cNOS) in the cells were determined. Compared with the Ang II-treated group, ICA can significantly raise the viability of EC, increase the activities of SOD, T-NOS and cNOS, increase the production of NO, enhance the capacity of scavenging superoxide anion radicals (O₂^·–) and hydroxyl radicals(·OH), and lower LDH leakage and iNOS activity. The results suggest that ICA can protect endothelial cells (ECV-304) from Ang II-induced injury.

Key words: Icariin, Icariin, Angiotensin II, Angiotensin II, Human umbilical vein endothelial cells line, Human umbilical vein endothelial cells line, Nitric oxide, Nitric oxide