氧化苦参碱磷脂复合物的制备及其体外抗乙肝病毒活性的研究

• 研究论文 • 上一篇

氧化苦参碱磷脂复合物的制备及其体外抗乙肝病毒活性的研究

王陆军, 岳鹏飞, 赵艳玲, 蔡培烈, 周旭, 袁海龙^*

1.中国人民解放军第302医院全军中药研究所, 北京 100039；
2.中药现代制剂教育部重点实验室, 江西南昌 330004;
3.汇仁集团, 上海 201203

收稿日期:2007-02-24 修回日期:2007-05-10 出版日期:2007-06-15 发布日期:2007-06-15
通讯作者: 袁海龙*

Preparation and study of anti-hepatitis B virus activity in vitro of oxymatrine phospholipid complex

Lu-Jun Wang, Peng-Fei Yue, Yan-Ling Zhao, Pei-Lie Cai, Xu Zhou, Hai-Long Yuan^*

1.302 Hospital of PLA and PLA Institute of Chinese Materia Medica, Beijing 100039, China;
2.Key Laboratory of Modern Preparation of TCM, Ministry of Education, Nanchang 330004, China;
3. HUIREN Group, Shanghai 201203, China

Received:2007-02-24 Revised:2007-05-10 Online:2007-06-15 Published:2007-06-15
Contact: Hai-Long Yuan*

摘要/Abstract

摘要：

目的制备苦参素磷脂复合物并对其体外抗乙肝病毒活性进行评价。方法利用四氢呋喃作为反应溶剂, 苦参素及磷脂溶于溶媒中, 真空除去有机溶剂得苦参素磷脂复合物。并以2.2.15 cell line为细胞模型, 分别对苦参素磷脂复合物的细胞毒性值和乙型肝炎表面抗原, 乙型肝炎e抗原, 乙型肝炎病毒DNA的抑制率进行了研究。结果制得的苦参素磷脂复合物中苦参素的含量为24.86%。苦参素磷脂复合物的细胞毒性值为250 μg×mL^–1。与苦参素相比, 苦参素磷脂复合物对2.2.15细胞的HBsAg, HBeAg, HBV-DNA值明显降低。结论苦参素磷脂复合物能够产生更有效的抗乙肝病毒活性。因此乙肝治疗中苦参素磷脂复合物有很强的抗病毒活性潜力。

关键词: 苦参素磷脂复合物, 苦参素磷脂复合物, 苦参素磷脂复合物, 2.2.15 Cell line, 2.2.15 Cell line, 2.2.15 Cell line, HBV-DNA, HBV-DNA, HBV-DNA

Abstract: Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved into the medium. Oxymatrine phospholipid complexs were prepared. The toxicity measurements and inhibitory effect on HBsAg, HBeAg, and HBVDNA of oxymatrine phospholipid complex in 2.2.15 cells were studied respectively. Results The content of oxymatrine in the phospholipids complexs prepared was 24.86% (W/W). The TCO of the oxymatrine phospholipid complexs was 250 μmol×L^–1. The inhibitory effect of HBsAg, HBeAg, HBV-DNA of 2.2.15 cells treated by oxymatrine phospholipid complex were higher than those of the oxymatrine. Conclusion Oxymatrine phospholipid complex can have stronger effective activity against hepatitis B virus compared with oxymatrine. So oxymatrine phospholipid complexs were showing its potential antiviral activity in hepatitis B treatment.

Key words: Oxymatrine phospholipid complex, Oxymatrine phospholipid complex, 2.2.15 Cell line, 2.2.15 Cell line, HBV-DNA, HBV-DNA

中图分类号:

R965

Supporting: ^*Corresponding author. Tel.: 86-10-66933324

王陆军, 岳鹏飞, 赵艳玲, 蔡培烈, 周旭, 袁海龙^*. 氧化苦参碱磷脂复合物的制备及其体外抗乙肝病毒活性的研究[J]. .

Lu-Jun Wang, Peng-Fei Yue, Yan-Ling Zhao, Pei-Lie Cai, Xu Zhou, Hai-Long Yuan^*

. Preparation and study of anti-hepatitis B virus activity in vitro of oxymatrine phospholipid complex[J]. .

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