RP-HPLC测定番茄红素微胶囊的含量

RP-HPLC测定番茄红素微胶囊的含量

卢定强^*, 陆晓云, 陆凯丰, 胡继军

南京工业大学制药与生命科学学院, 江苏南京 210009

收稿日期:2006-09-05 修回日期:2007-02-10 出版日期:2007-03-15 发布日期:2007-03-15
通讯作者: 卢定强*

RP-HPLC determination of lycopene in microcapsules

Ding-Qiang Lu^*, Xiao-Yun Lu, Kai-Feng Lu, Ji-Jun Hu

College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

Received:2006-09-05 Revised:2007-02-10 Online:2007-03-15 Published:2007-03-15
Contact: Ding-Qiang Lu*

摘要/Abstract

摘要： 目的建立反相高效液相色谱法测定番茄红素微胶囊中番茄红素含量的方法。方法采用反相高效液相色谱法, 色谱柱为美国Alltech Alltima C₁₈ (4.6mm×250mm, 5μm),流动相为甲醇-四氢呋喃-水(66∶30∶4,V/V/V), 流速为1.5mL·min^-1, 检测波长为472nm。结果线性范围为3.6～18μg·mL^-1, 相关系数r = 0.9998,平均回收率为99.81%～101.06% ,RSD为1.12%～1.83%, 日内和日间精密度的RSD分别在1.73%～2.54%、2.85%～3.34%之间。结论本文采用反相高效液相色谱法初步建立了番茄红素微胶囊中番茄红素的定量方法, 该方法准确, 快速, 重现性好。

关键词: 反相高效液相色谱法, 反相高效液相色谱法, 反相高效液相色谱法, 番茄红素, 番茄红素, 番茄红素, 微胶囊, 微胶囊, 微胶囊

Abstract: Aim A RP-HPLC method for determination of lycopene in microcapsules was established. Methods The HPLC assay was performed on an Alltima C₁₈(4.6 mm × 250 mm, 5 μm) column with a mixture of methanol-THF-water (66:30:4, V/V/V) as mobile phase at a flow rate of 1.5 mL·min^-1 and the UV detection wavelength was 472 nm. Results The linear range of lycopene was 3.6-18 μg·mL^-1, r = 0.9998, the average recovery was from 99.81% to 101.06% with RSD less than 1.83%. The RSD of intra-day and inter-day precision were less than 3.34%. Conclusion The method is simple, accurate and suitable for the determination of lycopene in microcapsules.

Key words: RP-HPLC, RP-HPLC, Lycopene, Lycopene, Microcapsules, Microcapsules

中图分类号:

Supporting:

Foundation item: Special Research Foundation of Ph.D. Study in University (20040291004) and Major Project of Chinese (National Programs for Fundamental Research(2003CB716000)

^*Corresponding author. Tel.: 86-25-83587334

卢定强^*, 陆晓云, 陆凯丰, 胡继军. RP-HPLC测定番茄红素微胶囊的含量[J]. .

Ding-Qiang Lu^*, Xiao-Yun Lu, Kai-Feng Lu, Ji-Jun Hu . RP-HPLC determination of lycopene in microcapsules[J]. .

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