丝裂霉素与DNA在固体石墨电极上的相互作用研究

丝裂霉素与DNA在固体石墨电极上的相互作用研究

郭明^*, 谭非, 胡润淮, 俞庆森

1.浙江林学院理学院化学系, 浙江临安 311300;
2.浙江大学理学院化学系, 杭州玉泉 310027;
3.浙江林学院生命科学学院中药系, 浙江临安 311300

收稿日期:2005-06-09 修回日期:2005-11-10 出版日期:2005-12-15 发布日期:2005-12-15
通讯作者: 郭明*

Interaction between Mitomycin C and DNA at Paraffined Graphite Electrode

GUO Ming^*, TAN Fei, HU Run-huai, YU Qing-sen

1.Department of Chemistry, Zhejiang Forestry University, Lin'an 311300, China;
2.Department of Chemistry, Zhejiang University, Hangzhou 310027, China;
3.Department of Traditional Chinese Medicine, Zhejiang Forestry University, Lin'an 311300, China

Received:2005-06-09 Revised:2005-11-10 Online:2005-12-15 Published:2005-12-15
Contact: GUO Ming*

摘要/Abstract

摘要： 应用循环伏安法(CV)研究了药物丝裂霉素(MC)与小牛胸腺DNA(ctDNA)在自制的浸蜡石墨电极上的相互作用。在Na2HPO4-NaH2PO4 溶液体系中, MC及MCctDNA反应物在电极上出现良好的还原-氧化峰。根据相应的电化学理论方程, 由实验数据求得了MC及MCDNA反应复合物的自由扩散系数及反应的表观速率常数。结果表明, 固体浸蜡石墨电极可方便、灵敏地用于研究丝裂霉素及与ctDNA相互作用的电化学特性并能用于求取相关参数。

关键词: 丝裂霉素, 丝裂霉素, 丝裂霉素, 小牛胸腺DNA, 小牛胸腺DNA, 小牛胸腺DNA, 循环伏安法, 循环伏安法, 循环伏安法, 浸蜡石墨电极, 浸蜡石墨电极, 浸蜡石墨电极

Abstract: Aim To investigate the electrochemical behaviors of Mitomycin C (MC) and its interaction with calf thymus DNA (ctDNA). Methods The cyclic voltammetry (CV) was carried out at a paraffined graphite electrode. Results MC showed a well-defined oxidation-reduction peak. As a result of reaction with ctDNA, the peak current of MC decreased apparently. According to corresponding electrochemical equations, the diffusion coefficient of both free and MC-DNA complex have been determined, and the heterogeneous rate constants were also obtained simultaneously. Conclusion The solid paraffined graphite electrode could be used to estimate parameters of the interaction between DNA and MC, and provide the convenient and sensitive analysis.

Key words: mitomycin C, mitomycin C, calf thymus DNA, calf thymus DNA, cyclic voltammetry, cyclic voltammetry, paraffined graphite electrode, paraffined graphite electrode

中图分类号:

R913

Supporting: Foundation item: Scientific Research Foundation of Zhejiang Forestry University.
^*Corresponding author. Tel.: 86-571-63732772; fax: 86-571-63748537

郭明^*, 谭非, 胡润淮, 俞庆森. 丝裂霉素与DNA在固体石墨电极上的相互作用研究[J]. .

GUO Ming^*, TAN Fei, HU Run-huai, YU Qing-sen.

Interaction between Mitomycin C and DNA at Paraffined Graphite Electrode

[J]. .

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