关键词: 荧光猝灭, 荧光猝灭, 荧光猝灭, 牛血清白蛋白, 牛血清白蛋白, 牛血清白蛋白, 加替沙星, 加替沙星, 加替沙星

Abstract: Aim To study the reaction mechanism between gati fl oxacin and bovine serum albumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used. Results The binding constants were determined from a double reciprocal Lineweaver- Burk curves at different pHs. The binding distance r under normal physiologi cal condition was obtained according to Foster theory of non-radiative energy transfer. The binding force between gatifloxacin and BSA was inferred by thermody namical coordination. Conclusion The interaction between gatifl oxacin and BSA seems to be strong and the main binding force is electrostatic fo rce.

Key words: fluorescence quenching, fluorescence quenching, bovine serum albumin, bovine serum albumin, gati floxacin, gati floxacin