RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量

RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量

孟湘明, 孟志云, 张亮, 窦桂芳^*

军事医学科学院野战输血研究所, 北京 100850

收稿日期:2003-11-14 修回日期:2004-11-10 出版日期:2004-12-15 发布日期:2004-12-15
通讯作者: 窦桂芳*

Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography

MENG Xiang-ming, MENG Zhi-yun, ZHANG Liang, DOU Gui-fang^*

Institute of Transfusion Medicine, Academy Military of Medical Science, Beijing 100850, China

Received:2003-11-14 Revised:2004-11-10 Online:2004-12-15 Published:2004-12-15
Contact: DOU Gui-fang*

摘要/Abstract

摘要： 目的建立反相高效液相色谱法用于同时测定他唑巴坦和头孢他啶的含量. 方法采用Zorbax 300SB-C18色谱柱, 流动相为甲醇-磷酸盐缓冲液 (pH = 5.6), 检测波长为220 nm. 结果他唑巴坦和头孢他啶分别在0.62-631.8 μg·mL^-1和 0.66-677.5 μg·mL^-1内呈线性关系, 平均加样回收率分别为98.8%-101.4%和99.1%-100.2%, 日内和日间精密度分别为0.2%-1.5%和0.1%-2.6%. 结论该法简单、精确、快速、重复性好, 可以满足其原料和制剂的质量控制要求.

关键词: 他唑巴坦, 他唑巴坦, 他唑巴坦, 头孢他啶, 头孢他啶, 头孢他啶, 反相高效液相色谱法, 反相高效液相色谱法, 反相高效液相色谱法

Abstract: Aim A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of ceftazidime and tazobactam in injectable powder. Methods Chromatography was carried out on Zorbax 300SB-C18 column using a mixture of methanol and aqueous solution of phosphate buffer (pH = 5.6) as mobile phase. The UV detection wavelength was 220 nm. Results The linear ranges of ceftazidime and tazobactam were 0.62-631.8 μg·mL^-1 and 0.66-677.50μg·mL^-1, respectively. The average recoveries were 98.8%-101.4% for ceftazidime, and 99.1%-100.2% for tazobactam. The RSD values of inter-day and intra-day assays were lower than 1.5% for ceftazidime and 2.6% for tazobactam. Conclusion This method is reproducible, simple, precise, and rapid for the quality control of ceftazidime and tazobactam in injectable powder.

Key words: ceftazidime, ceftazidime, tazobactam, tazobactam, RP-HPLC, RP-HPLC

中图分类号:

R917.01

R917.795

Supporting: ^*Corresponding author. Tel.: 86-10-66932951

孟湘明, 孟志云, 张亮, 窦桂芳^*. RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量[J]. .

MENG Xiang-ming, MENG Zhi-yun, ZHANG Liang, DOU Gui-fang^*. Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography[J]. .

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