反相高效液相色谱法测定狗血浆中番茄红素的浓度

反相高效液相色谱法测定狗血浆中番茄红素的浓度

王会娟, 李馨儒, 黄燕清, 刘艳^*

1.北京大学药学院药剂学系, 北京 100083;
2.北制药集团新药研发中心, 河北石家庄 050015

收稿日期:2004-06-21 修回日期:2004-11-10 出版日期:2004-12-15 发布日期:2004-12-15
通讯作者: 刘艳*

Determination of Lycopene Concentration in Dog Plasma by RP-HPLC

WANG Hui-juan, LI Xin-ru, Huang Yan-qing, LIU Yan^*

1.Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China
2.R & D Drug Centre, North China Pharmaceutical Group Corporation, Shijiazhuang 050015, China

Received:2004-06-21 Revised:2004-11-10 Online:2004-12-15 Published:2004-12-15
Contact: LIU Yan*

摘要/Abstract

摘要： 目的建立用反相高效液相色谱法测定狗血浆中番茄红素浓度的方法. 方法流动相为甲醇:乙腈:二氯甲烷(40:30:30, V/V), 检测波长472 nm, 柱温为室温, 流速为1.0 mL·min^-1. 结果标准曲线的线性范围在0.0124~0.496 μg·mL^-1, r = 0.9992. 平均萃取回收率为97.6%±4.2%, 日内和日间的RSD分别在1.52%~4.95%、2.31%~7.38%之间. 结论所建立的方法灵敏、快速、重现性好、选择性强.

关键词: 番茄红素, 番茄红素, 番茄红素, 血浆浓度, 血浆浓度, 血浆浓度, 反相高效液相色谱法, 反相高效液相色谱法, 反相高效液相色谱法

Abstract: Aim A method for determination of lycopene concentration in dog plasma was established. Methods RP-HPLC was used; the mobile phase wavelength of detection was 472 nm, the column temperature was ambient temperature, and the flow rate was 1.0 mL·min^-1. Results The standard curve was linear in the range from 0.012 4 to 0.496 μg·mL^-1 with r = 0.9992. The average extraction recovery was 97.6%±4.2%. The intra-day and inter-day RSD were 1.52%-4.95% and 2.31%-7.38%, respectively. Conclusion This method is sensitive, rapid, reproducible, and of good selectivity for the analysis of lycopene in dog plasma.

Key words: lycopene, lycopene, plasma concentration, plasma concentration, RP-HPLC, RP-HPLC

中图分类号:

R917.01

R927.14

Supporting: ^*Corresponding author. Tel.: 86-10-82801508

王会娟, 李馨儒, 黄燕清, 刘艳^*. 反相高效液相色谱法测定狗血浆中番茄红素的浓度[J]. .

WANG Hui-juan, LI Xin-ru, Huang Yan-qing, LIU Yan^* . Determination of Lycopene Concentration in Dog Plasma by RP-HPLC[J]. .

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