异核苷掺入寡核苷酸的合成及其与互补序列的结合研究

异核苷掺入寡核苷酸的合成及其与互补序列的结合研究

史继峰, 王占黎, 张亮仁^*, 张礼和

北京大学药学院, 北京100083, 中国

收稿日期:2003-07-15 修回日期:2004-02-10 出版日期:2004-03-15 发布日期:2004-03-15
通讯作者: 张亮仁*

Synthesis of Isonucleoside-Incorporated Oligonucleotides and Their Binding Abilities with Complementary Sequences

SHI Ji-feng, WANG Zhan-li, ZHANG Liang-ren^*, ZHANG Li-he

School of Pharmaceutical Sciences, Peking University, Beijing 100083, China

Received:2003-07-15 Revised:2004-02-10 Online:2004-03-15 Published:2004-03-15
Contact: ZHANG Liang-ren*

摘要/Abstract

摘要： 目的　合成异核苷掺入寡核苷酸并考察它们与互补序列的结合能力。方法　采用DNA固相合成仪合成寡核苷酸,通过热变性实验考察双链的稳定性。结果　合成了四条单异核苷掺入的寡核苷酸, 热变性实验结果发现异核苷的引入降低了双链的稳定性, 当异核苷处于寡核苷酸链的中央时, 这种影响更为明显。异核苷6'-OH处于游离和烯丙基保护状态时的结果没有显著差异。结论　寡核苷酸中的异核苷使链发生扭曲,从而导致双链稳定性降低。

关键词: 异核苷, 异核苷, 寡核苷酸, 寡核苷酸, 杂交, 杂交, 热变性, 热变性

Abstract: Aim To synthesize isonucleoside-incorporated ol igonucleotides and investigate their binding abilities with complementary sequen ces. Methods The synthesis was performed on DNA synthesizer, and the binding behavior was investigated by thermal denaturation studies. Results Four kinds of single isonucleoside containing oligonucleoti des were synthesized. The results of thermal denaturation showed that the existe nce of isonucleoside decreased the stability of duplex, and the effect was more obvious when the isonucleoside was in the middle of the sequence. No obvious dif ference was observed when 6'-OH of isonucleoside was free or was protected by allyl group. Conclusions The existence of isonucleoside in oli gonucleotide makes chain twist and decreased the stability of duplex.

Key words: isonucleoside, isonucleoside, nucleotides, nucleotides, hybridization, hybridization, ther mal denaturation, ther mal denaturation

中图分类号:

R916.41

R916.696

Supporting: Foundation item: National Natural Science Foundation of China (20072002) and The Key Laboratory of Bioorganic Phosphorus Chemistry , Ministry of Education.
^*Corresponding author. Tel.: 010-82801570

史继峰, 王占黎, 张亮仁^*, 张礼和. 异核苷掺入寡核苷酸的合成及其与互补序列的结合研究[J]. .

SHI Ji-feng, WANG Zhan-li, ZHANG Liang-ren^*, ZHANG Li-he . Synthesis of Isonucleoside-Incorporated Oligonucleotides and Their Binding Abilities with Complementary Sequences[J]. .

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