大麻RAPD研究的方法学探讨

大麻RAPD研究的方法学探讨

宋书娟, Robert.C.Clarke, 邵宏

1.北京大学医学部生物遗传教研室, 北京 100083;
2.国际大麻协会, 75007 荷兰

收稿日期:2001-02-12 修回日期:2001-03-20 出版日期:2001-06-15 发布日期:2001-06-15

An Effective Procedure for the RAPD Analysis of Hemp Cannabis sativa L.

Song Shujuan, Robert. C.Clarke, Shao Hong

1. Department of Biology and Genetics, Peking University Health Science Center, Beijing 100083;
2. International Hemp Association. P. O. Box 75007, 1070AA Amsterdam, The Netherland

Received:2001-02-12 Revised:2001-03-20 Online:2001-06-15 Published:2001-06-15

摘要/Abstract

摘要： 中国有丰富的大麻资源。目前, 在我国对于大麻的研究多集中在形态和化学成分等方面, 而分子遗传水平的研究至今尚未见报道。RAPD分子标记技术是适合于大麻分子水平研究的首选方法。本文以采集多年的大麻植物干标本的叶为研究材料, 分别对Mg²⁺, dNTPs, Taq DNA 酶的浓度, 和退火温度和退火时间以及反应循环数等条件进行了检测, 得到了适合大麻RAPD研究的理想条件。实验结果表明, 用干标本来进行一定的分子遗传研究是可行的, 且结果也相对稳定。

关键词: 大麻, 干标本, RAPD技术

Abstract: China has a great resource of Cannabis. Research on the taxonomy and morphology of Chinese Cannabis has been carried out, but so far no molecular genetic research has been published. Random amplified polymorphic DNA (RAPD) is a suitable technique for molecular genetic research on Cannabis. In this experiment, using Cannabis herbarium specimens as a source of genetic materials, the correlative conditions of the Polymerase chain reaction (PCR), (i.e., gradient density of Mg²⁺, dNTPs, Taq DNA polymerase, anneal temperature, anneal time and reaction cycles) were examined separately. An effective procedure for the RAPD analysis of Cannabis was obtained.

Key words: Cannabis, Cannabis, Herbarium specimen, Herbarium specimen, Random amplified polymorphic DNA, RAPD, Random amplified polymorphic DNA, RAPD

Supporting:

宋书娟, Robert.C.Clarke, 邵宏. 大麻RAPD研究的方法学探讨[J]. .

Song Shujuan, Robert. C.Clarke, Shao Hong . An Effective Procedure for the RAPD Analysis of Hemp Cannabis sativa L.[J]. .