槲寄生抗血小板激活因子活性成分的研究

槲寄生抗血小板激活因子活性成分的研究

管增伟, 刘雪辉, 刘海新, 崔育新^*

北京医科大学医药卫生分析中心, 北京 100083

收稿日期:2000-01-26 修回日期:2000-03-28 出版日期:2000-06-15 发布日期:2000-06-15
通讯作者: 崔育新*

A Novel Platelet-Activating Factor Antagonist Isolated from a Chinese Herbal Drug Viscum coloratum

Guan Zengwei, Liu Xuehui, Liu Haixin, Cui Yuxin^*

Medical and Pharmaceutical Analysis Center Beijing Medical University, Beijing 100083

Received:2000-01-26 Revised:2000-03-28 Online:2000-06-15 Published:2000-06-15
Contact: Cui Yuxin*

摘要/Abstract

摘要： 本文以血小板激活因子(PAF)和二磷酸腺苷(ADP)诱导的血小板聚集试验为检测指标, 追踪观测了中草药槲寄生对PAF和ADP诱导的血小板聚集的影响。从槲寄生乙酸乙酯提取物中分离得到的化合物高圣草素7-O-β-D-葡萄糖苷, 体外试验证实对PAF诱导的血小板聚集有明显的抑制作用, 而对ADP诱导的血小板聚集无显著性抑制作用。提示高圣草素7-O-β-D-葡萄糖苷是槲寄生抗PAF的有效活性成分。

关键词: 槲寄生, 高圣草素-7-O-β-D-葡萄糖苷, 血小板激活因子, 血小板激活因子拮抗剂, 血小板聚集

Abstract: A novel natural platelet-activating factor (PAF) antagonist was isolated from a Chinese medicinal. herb Viscum coloratum and its structure was identified as ho moeriodictyol-7-O-β-D-glycoside, which existed in ethyl acetate extract fraction. At the final concentration of 10^-5 mol·L^-1, it could significantly inhibit human and rabbit platelet aggregation induced by PAF, with IC50 being 8.0×10^-7 mol·L^-1, but had no inhibitory effect on platelet aggregation induced by ADP.

Key words: Viscum coloratum, Platelet-activating factor antagonist, Viscum coloratum, Platelet-activating factor antagonist, Homoeriodictyol-7-O-β-D-glycoside, Homoeriodictyol-7-O-β-D-glycoside, Platelet aggregation, Platelet aggregation

Supporting: ^*Correspondence to: Cui Yuxin, Medical and Pharmaceutical Analysis Center, Beijing Medical University, Beijing 100083, P.R.China

管增伟, 刘雪辉, 刘海新, 崔育新^*. 槲寄生抗血小板激活因子活性成分的研究[J]. .

Guan Zengwei, Liu Xuehui, Liu Haixin, Cui Yuxin^*. A Novel Platelet-Activating Factor Antagonist Isolated from a Chinese Herbal Drug Viscum coloratum[J]. .

[1]	周北斗, 王欣, 翁智敏, 黄堡城, 马泽通, 于博, 阮丽琴, 胡栋宝. 氧杂蒽酮的合成和抗肿瘤、抑制酪氨酸酶和抑制血小板聚集活性研究[J]. 中国药学(英文版), 2019, 28(4): 247-256.
[2]	管增伟, 王银叶, 杨秀伟, 崔育新. 高圣草素-7-O-β-D-葡萄糖苷抑制血小板活化因子诱导血小板聚集的机理探讨[J]. , 2001, 10(1): 42-44.
[3]	许天林^, 蒋巡天^, 华维一, 倪沛洲, 裴咏梅^. 非肽类纤维蛋白原受体拮抗剂(Ⅱ): N-取代-O-对甲脒苯氧乙基-L-酪氨酸甲酯的合成与抗血小板聚集活性研究[J]. , 1999, 8(4): 201-206.
[4]	迪丽努尔·莎比托夫, 赵明, 王超, 彭师奇^*. YIGSK和含RGD多肽的抗血小板聚集作用[J]. , 1998, 7(3): 127-129.
[5]	谢梅林, 陆群, 顾振纶. β-榄香烯对血小板聚集的抑制作用及其机制分析[J]. , 1998, 7(2): 112-114.
[6]	丁忠仁, 黄守坚, 孙家钧. 补体激活对血小板聚集的影响[J]. , 1998, 7(1): 18-24.
[7]	吴俊芳^*, 刘天培. 小檗碱对脑缺血兔血浆血栓素B₂和6-酮前列腺素F_1α的影响[J]. , 1998, 7(1): 33-37.
[8]	包丽华, 罗大力, 杨宝峰, 何树桩, 李文汉. 小檗胺对人中性粒细胞PAF生成和PAF诱导的兔血小板聚集的抑制作用[J]. , 1997, 6(1): 37-40.
[9]	王福军, 张世玲. BM-13505抗栓作用及机理研究[J]. , 1995, 4(2): 67-74.
[10]	赵明, 彭师奇, 蔡孟深, 唐朝枢, 李长龄, 于学敏, 王振南. RGDS的抗血小板聚集及舒血管效应[J]. , 1993, 2(1): 18-23.