C57小鼠发育过程中海马NGF基因转录水平的研究

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C57小鼠发育过程中海马NGF基因转录水平的研究

刘晓军, 吴梧桐^*, 张林元, 高向东

1. 中国药科大学生物制药学院, 南京 210009;
2. 南京军区后勤军事医学研究所, 南京 210002

收稿日期:1999-07-07 修回日期:1999-11-08 出版日期:2000-06-15 发布日期:2000-06-15
通讯作者: 吴梧桐*

Study on the Gene Transcription Level of Hippocampus NGF in C57 Mouse Development

Liu Xiaojun, Wu Wutong^*, Zhang Linyuan, Gao Xiangdong

1. School of Biopharmaceutics, China Pharmaceutical University Nanjing 210009;
2. Nanjing Institute of Military Medicine, Nanjing 210002

Received:1999-07-07 Revised:1999-11-08 Online:2000-06-15 Published:2000-06-15
Contact: Wu Wutong*

摘要/Abstract

摘要： 采用半定量RT-PCR法测定了1,6,12月龄C57小鼠海马β NGF mRNA水平, 将β肌动蛋白基因作为内标。结果表明, 6, 12月龄两组间无显著性差异, 但均显著低于1月龄组, 说明C57小鼠发育过程中其海马β NGF基因转录水平明显降低。另外, 发现C57小鼠海马β NGF基因第294位碱基为C, 与普通小鼠的T不同, 但这种碱基差异并不影响氨基酸组成。

关键词: NGF, β-肌动蛋白, RT-PCR, 基因转录, C57小鼠, 发育

Abstract: β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.

Key words: NGF, β-actin, RT-PCR, NGF, β-actin, RT-PCR, Gene transcription, Gene transcription, C57 mouse, C57 mouse, Development, Development

Supporting:

刘晓军, 吴梧桐^*, 张林元, 高向东. C57小鼠发育过程中海马NGF基因转录水平的研究[J]. .

Liu Xiaojun, Wu Wutong^*, Zhang Linyuan, Gao Xiangdong. Study on the Gene Transcription Level of Hippocampus NGF in C57 Mouse Development[J]. .

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