人心律失常相关基因Kv1.5和Kv4.2在蟾蜍卵母细胞上的表达

人心律失常相关基因Kv1.5和Kv4.2在蟾蜍卵母细胞上的表达

许东晖, 许实波, 王至国, Stanley Nattel

1.中山大学药学系药理研究室, 广州 510275
2.Research Center, Montreal Heart Institute, HIT 1C8, Canada

收稿日期:1998-10-06 修回日期:1999-09-28 出版日期:1999-12-15 发布日期:1999-12-15

The Expression of Human Arrhythmic Related Gene Kv1.5 and Kv4.2 on Xenopus Oocytes

Xu Donghui, Xu Shibo, Wang Zhiguo, Stanley Nattel

1.Research Section of pharmacology Department of pharmacy Zhongshan University Guangzhou 510275;
2.Research Center Montreal Heart Institute, HIT 1C8 Canada

Received:1998-10-06 Revised:1999-09-28 Online:1999-12-15 Published:1999-12-15

摘要/Abstract

摘要： 本文采用基因克隆、膜片钳和微注射技术, 分别将人的心律失常相关基因Kv1.5和Kv4.2c DNA转录入cRNA, 将Kv1.5c RNA和Kv4.2c RNA分别注射蟾蜍卵母细胞(Xenopus oocytes), 分别在蟾蜍孵母细胞上获得纯净、单一的超速延迟性整流钾电流(IKur, ultrarapid delayed rectifier K⁺ currsent)和瞬时外向钾电流(Ito, transient outward K⁺current)表达, 克服以往在筛选评价抗心律失常药物时, 人新鲜心肌细胞取材困难, 以及多种电流在细胞膜上共同表达等缺点, 从而建立筛选评价Ⅲ类抗心律失常药物的先进药理模型。

关键词: 心律失常基因, Kv1.5, Kv4.2, 蟾蜍卵母细胞, 药理模型

Abstract: By techniques of gene clone, microinjection and patch-clamp, human arrhythmicrelated gene Kv1 .5 and Kv4.2 were translated into cRNA, and then injecte4 into Xenopus oocytes, respectively. A pure and single K⁺ current of ultrarapid delayed rectifier K⁺ current (IKur) or transientoutward K⁺ current (Ito) was respectively detected on Xenopus oocytes. This is a modern pharmacological model for evaluating class III antiarrhythmic drugs. It can overcome many defects of previousmethods for evaluating antiarrhythmic drugs, such as lacking human fresh cardiac muscle cells asmaterial, and co-expression of many currents on cell membrane.

Key words: Arrhythmic gene, Kv1.5, Kv4.2, Xenopus oocyte, Pharmacological model

Supporting:

许东晖, 许实波, 王至国, Stanley Nattel. 人心律失常相关基因Kv1.5和Kv4.2在蟾蜍卵母细胞上的表达[J]. .

Xu Donghui, Xu Shibo, Wang Zhiguo, Stanley Nattel. The Expression of Human Arrhythmic Related Gene Kv1.5 and Kv4.2 on Xenopus Oocytes[J]. .