苄基四氢巴马汀对豚鼠心室肌单细胞动作电位及延迟整流钾电流两种成分的影响

苄基四氢巴马汀对豚鼠心室肌单细胞动作电位及延迟整流钾电流两种成分的影响

阎升, 李新华, 姚伟星, 夏国瑾, 江明性, 黄文龙, 黄枕亚, 彭司勋

1. 同济医科大学药理教研室, 武汉430030;
2. 中国药科大学药化研究室, 南京210009

收稿日期:1997-10-10 修回日期:1998-03-09 出版日期:1998-12-15 发布日期:1998-12-15

The Effect of Benzyltetrahydropalmatine(BTHP) on Action Potentials and the Two Components of Delayed Rectifying Potassium Currents in Guinea Pig Ventricular Myocytes

Sheng Yah, Xin-Hua Li, Wei-Xing Yao, Guo-Jin Xia, Ming-Xing Jiang, Wen-Long Huang, Zhen-Ya Huang, Si-Xun Peng

1. Department of Pharmacology, TongJi Medical University, Wuhan 430030;
2. Division of Medical Chemistry, China Phnrmoceuncdt University, Nanfing 210009

Received:1997-10-10 Revised:1998-03-09 Online:1998-12-15 Published:1998-12-15

摘要/Abstract

摘要： 苄基四氢巴马汀为四氢巴马汀的衍化物, 本文采用标准膜片钳全细胞记录技术, 研究了苄基四氢巴马汀对豚鼠心室肌单细胞动作电位及延迟整流钾通道(Ik)两种成分(Iks和Ikr)的选择性作用。结果表明: (1) 30 μmol·L^{- 1}苄基四氢巴马汀可显著延长无钙细胞外液条件下动作电位的APD90 (从143±16 ms 到184±21 ms, P<0101, n = 5), 而对RP, APA 无明显影响, 其对 APD90的延长作用不依赖于胞外钙。(2) 苄基四氢巴马汀对延迟整流钾通道两种成分均有不同程度的阻滞作用, 其阻滞Ikr的IC50 (半数抑制浓度) 为719 μmol·L^{- 1}, 阻滞 Iks的 IC50为2214 μmol·L^{- 1}, 可见苄基四氢巴马汀对 Ik 的阻滞作用无选择性。

关键词: 苄基四氢巴马汀, 膜片钳技术, 延迟整流钾电流, 心室肌细胞

Abstract: The effects of BTHP on Ca²⁺-independent action potential and the two components of delayed rectifier potassium currents were studied in guinea pig single ventricular myocytes by using whole cell patch clamp technique. BTHP 30 μmol·L^{- 1} significantly prolonged APD90 from 143±16 ms to 184±21 ms (P<0.01, n = 5) without affecting either the RP or APA , and the APD prolonging effects of BTHP were independent of extracellular Ca²⁺. BTHP inhibited both Ikr (IC50 = 719μmol·L^{- 1}) and Iks (IC50 = 2214 μmol·L^{- 1}) in a concentration-dependent fashion. The results demon-strated that BTHP had no obvious selectivity for Ikr and Iks.

Key words: Benzyltetrahydropalmatine, Patch clamp technique, Delayed rectifier potassium channel, Ventricular myocytes

Supporting: ^*Project was supported by National Natural Science Foundation of China (No. 39370795).

阎升, 李新华, 姚伟星, 夏国瑾, 江明性, 黄文龙, 黄枕亚, 彭司勋. 苄基四氢巴马汀对豚鼠心室肌单细胞动作电位及延迟整流钾电流两种成分的影响[J]. .

Sheng Yah, Xin-Hua Li, Wei-Xing Yao, Guo-Jin Xia, Ming-Xing Jiang, Wen-Long Huang, Zhen-Ya Huang, Si-Xun Peng. The Effect of Benzyltetrahydropalmatine(BTHP) on Action Potentials and the Two Components of Delayed Rectifying Potassium Currents in Guinea Pig Ventricular Myocytes[J]. .

[1]	李泱^*, 傅丽英, 姚伟星, 夏国瑾, 江明性. 苄基四氢巴马汀和甲氧胺对豚鼠心室肌细胞延迟整流钾电流阻滞作用[J]. , 2002, 11(3): 105-109.
[2]	李新华, 阎升, 姚伟星, 夏国瑾, 江明性, 黄文龙, 彭司勋. IQ₂₃对豚鼠心室肌细胞复极和延迟整流钾电流两种成分的作用[J]. , 1999, 8(1): 48-52.