灯盏花素抑制大鼠脑线体脂质过氧化的作用机制

灯盏花素抑制大鼠脑线体脂质过氧化的作用机制

陈小夏, 何冰, 陈一岳

广东药学院药理教研室, 广州 510224

收稿日期:1998-04-24 修回日期:1998-06-08 出版日期:1998-12-15 发布日期:1998-12-15

Mechanisms of Inhibitory Effects of Breviscapine on Lipid Peroxidation in Rat Brain Mitochondria

Xiao-Xia Chen, Bing He, Yi-Yue Chen

Department of Pharmacology, Guangdong College of Pharmacy, Guangzhou 510224

Received:1998-04-24 Revised:1998-06-08 Online:1998-12-15 Published:1998-12-15

摘要/Abstract

摘要： 本文研究了灯盏花素抑制脂质过氧化的作用机制。大鼠脑线粒体脂质过氧化物用硫代巴比妥酸比色法测定。灯盏花素与铁的螯合活性用差示光谱法测定。黄嘌呤-黄嘌呤氧化酶(Xan-XO)体系产生的超氧阴离子自由基(O2^·-)及FeSO4-H2O2体系产生的羟自由基(·OH)用比色法测定。结果表明: 灯盏花素能有效地抑制Xan-XO和FeSO4-H2O2诱导的脑线粒体脂质过氧化反应, 其IC50分别为93.01和62.18 μmol·L^-1。灯盏花素也能清除Xan-XO体系产生的O2^·-和FeSO4-H2O2体系产生的·OH, 其IC50分别为32.63和20.22 μmol·L^-1。灯盏花素还具有螯合Fe²⁺的活性。由此可见, 灯盏花素是在氧自由基与线粒体膜的反应中(1)·OH的形成(通过与Fe²⁺螯合)(2)脂质过氧化的启动(通过清除O2^·-和·OH)两个环节抑制脂质过氧化反应的。清除氧自由基和与Fe²⁺螯合是灯盏花素抑制脑线粒体脂质过氧化的作用机制。

关键词: 灯盏花素, 大鼠脑线粒体, 脂质过氧化, 氧自由基, 螯合

Abstract: The mechanisms by which breviscapine (Bre) inhibits the lipid preoxidation in rat brain mitochondria were investigated. The mitochondrial lipid peroxidation of rat brain induced by oxygen free radical was measured by thiobarbituric acid spectrophotometry. The chelating activities of Bre for Fe²⁺ were tested by differential spectrum. Superoxide anion (O2^·-) from xanthine-xanthine oxidase (Xan-XO) system and hydroxyl radical (·OH) from FeSO4-H2O2 system were determined with spectrophotometry. It was found that Bre could effectively inhibit the lipid peroxidation of brain mitochondria induced by free radicals driven from Xan-XO and FeSO4-H2O2 system. The IC50 of Bre were 93.01 μmol·L-1 for Xan-XO system and 62.18 μmol·L^-1 for FeSO4-H2O2 system. Bre also scavenged O2^·- and ·OH produced by Xan-XO and FeSO4-H2O2 systems. The IC50 of Bre were 32.63 μmol·L^-1 for O2^·- and 20.22 μmol·L^-1 for ·OH. Furthermore, the chelating Fe²⁺ activity of Bre was shown. It may be concluded that Bre inhibited lipid peroxidation at different stages of the reaction of oxygen free redial with the mitochondria membrane: (1) the formation of ·OH; (2) the initiation of the lipid peroxidation, by chelating Fe²⁺ and scavenging O2^·- as well as ·OH. The scavenging oxygen free radicals and chelating iron are the mechanisms of inhibitory effect of Bre on lipid peroxidation.

Key words: Breviscapine, Brain mitochondria, Lipid peroxidation, Oxygen free radical, Chelator

Supporting:

陈小夏, 何冰, 陈一岳. 灯盏花素抑制大鼠脑线体脂质过氧化的作用机制[J]. .

Xiao-Xia Chen, Bing He, Yi-Yue Chen. Mechanisms of Inhibitory Effects of Breviscapine on Lipid Peroxidation in Rat Brain Mitochondria[J]. .

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