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不同产地和不同采收期黄岑中黄酮类成分的高效液相色谱法定量分析

张永煜, 郭允珍, 上田博之, 针谷羲弘, 恩田政行, 桥本和则, 池谷幸信, 罔田稔, 丸野政雄   

  1. 1. 沈阳药科大学, 沈阳 110015;
    2. 昭和药科大学, 东京194, 日本;
    3. 北里大学药学部, 东京 108, 日本;
    4. 津村中央研究所, 茨城300-11, 日本
  • 收稿日期:1997-03-29 修回日期:1997-06-24 出版日期:1998-09-15 发布日期:1998-09-15

Quantitative Analysis of Flavonoids in Scutellariae Radix of Different Sources and Seasonal Variation by HPLC

Yong-Yu Zhang, Yun-Zhen Guo, Hiroyuki Ageta, Yoshihiro Harigaya, Masayuki Onda, Kadsunori Hashimoto, Yukinobu Ikeya, Minoru Okada, Masao Maruno   

  1. 1. Shenyang Pharmaceutical University, Shenyang 110015;
    2. Showa College of Pharmaceutical Sciences, Machida, Tokyo 194, Japan;
    3. School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan;
    4. Tsumura Central Laboratories, Tsumura and Co., Ibaraki 30011, Japan
  • Received:1997-03-29 Revised:1997-06-24 Online:1998-09-15 Published:1998-09-15

摘要: 用反相ODS, 磷酸缓冲溶液和甲醇(68:321:1)作为流动相, 对中国七个省产的黄芩和辽宁同一产地不同采收期(1990-1992)黄芩中黄芩甙、黄芩素、汉黄芩甙和汉黄芩素四种主要的有效成分进行了定量分析。实验结果表明, 河北承德地区野生黄芩中四种成分的含量最高。辽宁凌源野生黄芩的最佳采收期应为8月末。

关键词: 黄芩根, 黄芩, 不同产地, 不同采收期

Abstract: The seasonal and source variations of flavonoid contents in Scutellariae Radix were investigated by using the materials collected at the same place in Liaoning Province, China, during three years, 1990-1992, and in seven other provinces in China. Four principal flavonoids in the plant roots, i.e., baicalin, baicalein, wogonin-7-O-glucuronide and wogonin were analyzed by using a reversed-phase chromatographic system with a chemically bonded ODS silica gel column and phosphate buffer-methanol (68:32 and 1:1) as mobile phase. The contents of the four compounds combined in the herb collected at Chengde, Hebei Province are the highest. The results show that the best time for harvesting the roots in Liaoning Province is the end of August.

Key words: Scutellariae Radix, Scutellaria baicalensis Georgi, Different sources, Different harvest times

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