小鼠βNGF抗体纯化和活性鉴定

小鼠βNGF抗体纯化和活性鉴定

刘晓军, 吴梧桐, 高向东

中国药科大学生化教研室, 南京 210009

收稿日期:1996-12-26 修回日期:1997-10-21 出版日期:1998-03-15 发布日期:1998-03-15

Purification and Bioassay of Mouse β NGF Antibody

Xiao-Jun Liu, Wu-Tong Wu, Xiang-Dong Gao

Department of Biochemistry, China Pharmaceutical University, Nanjing 210009

Received:1996-12-26 Revised:1997-10-21 Online:1998-03-15 Published:1998-03-15

摘要/Abstract

摘要：

本文报道以小鼠βNGF为抗原免疫家兔,制备βNGF抗血清,用于Western印迹法,测定结果βNGF在分子量约14 kDa处显一条带。亲和层析纯化抗血清得到βNGF抗体。采用鸡胚背根神经节培养法测定了βNGF及其抗体的生物学活性。

关键词: βNGF抗体, 亲和层析, Western印迹法, 鸡胚背根神经节

Abstract: Anti-β NGF antiserum was prepared by immunizing rabbits with purified mouse β NGF. It presented immunopositivity to β NGF on Western blot, resulting in a single band approximately at the molecular weight of 14 kDa. The antiserum was further purified into anti-β NGF IgG by affinity chromatography. Chicken embryonic dorsal root ganglia (DRG) were cultured to test the biological activities of β NGF and its antibody.

Key words: Anti-β NGF antibody, Affinity chromatography, Western blot, Chicken embryonic DRG

Supporting: ^*This project was supported by Funds of Natural Science of Jiangsu Province (1995).

刘晓军, 吴梧桐, 高向东. 小鼠βNGF抗体纯化和活性鉴定[J]. .

Xiao-Jun Liu, Wu-Tong Wu, Xiang-Dong Gao. Purification and Bioassay of Mouse β NGF Antibody[J]. .