汗防已碱和1,6-二磷酸果糖对大鼠局部脑缺血模型的作用

汗防已碱和1,6-二磷酸果糖对大鼠局部脑缺血模型的作用

董志, 薛春生, 周歧新

重庆医科大学生化药理研究室, 重庆 630046

收稿日期:1996-02-26 修回日期:1996-12-05 出版日期:1997-03-15 发布日期:1997-03-15

Protective Effect of Tetrandrine and Fructose-1,6-diphosphate on the Model of Focal Cerebral Ischemia in Rats

Zhi Dong, Chun-Sheng Xue, Qi-Xin Zhou

Department of Biochemical Pharmacology; Chongqing University of Medical Sciences; Chongqing 630046

Received:1996-02-26 Revised:1996-12-05 Online:1997-03-15 Published:1997-03-15

摘要/Abstract

摘要： 以大鼠大脑中动脉阻塞24 小时后的梗塞面积和体积为指标, 试验了汉防己碱和1,6-二磷酸果糖对大鼠局部脑缺血的保护作用。在大脑中动脉阻塞后, 汉防己碱7.5, 12.0 和15.0 mg·kg^-1以及1, 6-二磷酸果糖200 和350 mg·kg^-1分别立即腹腔给药, 以剂量依赖方式明显减少大鼠脑的梗塞面积和体积。MK801 2.0 mg·kg^-1亦能减少梗塞面积和体积。汉防己碱和1, 6-二磷酸果糖联合用药所产生的保护作用比任何一药物单用时的保护作用都好, 提示汉防己碱和1,6-二磷酸果糖有协同作用。在大脑中动脉阻塞后1 小时和2 小时给药, 汉防己碱和1, 6-二磷酸果糖仍有脑缺血保护作用, 但在梗塞后3 小时给药则未见任何保护作用。提示中风或脑缺血后, 既非5 分钟即不可救药, 但亦应尽早用药。实验结果表明汉防己碱和1, 6-二磷酸果糖可能是有希望的脑缺血保护剂。

关键词: 汉防己碱, 1,6-二磷酸果糖, MK801, 局部脑缺血, 大脑中动脉阻塞, 脑梗塞

Abstract: The effect of tetrandrine (Tet) on the infarction area and volume of rat brain induced by middle cerebral artery occlusion (MCAO) was investigated. The treatment with Tet 7.5, 12.0 or 15.0 mg·kg^-1, or with fructose-1,6-diphosphate (FDP) 200 and 350 mg·kg^-1 ip immediately after MCAO, respectively, significantly reduced the infarction area and volume in a dose dependent manner. MK801 and FDP also displayed a protective effect on brain ischemia. A combination of Tet and FDP administered immediately after MCAO, produced a more potent protective effect than those treated with Tet or FDP alone. When Tet or FDP was administered 1 h and 2 h after MCAO, respectively, they could still significantly reduce the infarction area and volume of brain tissue. But, there was no significant protective effect when these two compounds were given 3 h after MCAO.

Key words: Tetrandrine, Fructose-1,6-diphosphate, MK801, Focal cerebral ischemia, Middle cerebral artery occlusion, Cerebral infarction

Supporting: Supported by the Foundation of Science and Technology Committee of Sichuan Province.

董志, 薛春生, 周歧新. 汗防已碱和1,6-二磷酸果糖对大鼠局部脑缺血模型的作用[J]. .

Zhi Dong, Chun-Sheng Xue, Qi-Xin Zhou. Protective Effect of Tetrandrine and Fructose-1,6-diphosphate on the Model of Focal Cerebral Ischemia in Rats[J]. .