贝母中浙贝甲素和浙贝乙素的柱前衍生化高效液相色谱测定

贝母中浙贝甲素和浙贝乙素的柱前衍生化高效液相色谱测定

李文彦, 毕开顺^*, 乔延江, 宁黎丽, 罗旭

1. 沈阳药科大学, 沈阳 110015;
2. 长春地质学院, 长春 130026

收稿日期:1996-05-27 修回日期:1996-07-01 出版日期:1996-12-15 发布日期:1996-12-15
通讯作者: 毕开顺*

A Pre-column Derivatization HPLC Method for the De-termination of Peimine and Peiminine in Bulbus Fritillar-iae

Wen-Yan Li, Kai-Shun Bi^*, Yan-Jiang Qiao, Li-Li Ning, Xu Luo

1. Shenyang Pharmaceutical University, Shenyang 110015;
2. Changchun Universyty of Earth Science, Changchun 130026

Received:1996-05-27 Revised:1996-07-01 Online:1996-12-15 Published:1996-12-15
Contact: Kai-Shun Bi*

摘要/Abstract

摘要： 本文提出了一个用于贝母生物碱浙贝甲素和浙贝乙素测定的柱前衍生化反相高效液相色谱法。浙贝甲素和浙贝乙素苯甲酸酯的峰面积Y1, Y2与该二生物碱的重量X1, X2分别在0.504~3.126 μg和0.520~3.28 μg范围内呈良好的线性关系。回归方程为Y1 = -3.83×10³ + 1.33×10⁵X1, r = 0.998, Y2 = -7.86×10² + 6.33×10⁴X2, r = 0.995; 平均回收率分别为98.0% (n = 5, RSD = 2.1%)和101.0% (n = 5, RSD = 4.1%)。本研究测定了15种不同产地、不同种的中药贝母中浙贝甲素和渐贝乙素的含量。结果表明, 浙贝甲素和浙贝乙素是贝母属植物鳞茎中的主要化学成分。

关键词: 浙贝甲素, 浙贝乙素, 柱前衍生化, 反相高效液相色谱法

Abstract: A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized, the calibration curve is Y1 = -3.83×10³ + 1.33×10⁵X1, r = 0.998, for P and Y2 = -7.86×10² + 6.33×10⁴X2, r = 0.995 for PE, where Y is the peak area and X is the weight of the alkaloid; the average recovery is 98.0% (n = 5, RSD = 2.1%) for P and 101.0% (n = 5, RSD = 4.1%) for PE, the linear range is from 0.504 μg to 3.126 μg for P and from 0.520 μg to 3.328 μg for PE, respectively. Results of the determination of the two alkaloids in several samples of different Fritillaria species from various parts of the country are presented. The results suggest that P and PE are two major chemical constituents in bulbs of different Fritillaria species, and that the method developed is generally applicable to the determination of the hydroxy group on aliphatic fused ring systems without steric hindrance.

Key words: Peimine (P), Peiminine (PE), Derivatization, HPLC

Supporting:

李文彦, 毕开顺^*, 乔延江, 宁黎丽, 罗旭. 贝母中浙贝甲素和浙贝乙素的柱前衍生化高效液相色谱测定[J]. .

Wen-Yan Li, Kai-Shun Bi^*, Yan-Jiang Qiao, Li-Li Ning, Xu Luo. A Pre-column Derivatization HPLC Method for the De-termination of Peimine and Peiminine in Bulbus Fritillar-iae[J]. .

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