鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶活性的影响

鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶活性的影响

周建政^*, 吴葆杰, 张岫美

山东医科大学基础部药理学教研室, 济南 250012;

收稿日期:1994-11-21 修回日期:1996-05-23 出版日期:1996-09-15 发布日期:1996-09-15
通讯作者: 周建政*

Effect of Fish Oil on β-Adrenoceptors and the Activity of Adenylate Cyclase on Myocardial Membrane in Rats

Jian-Zheng Zhou^*, Bao-Jie Wu, Xiu-Mei Zhang

Department of Pharmacology; Shandong Medical University; Jinan 250012

Received:1994-11-21 Revised:1996-05-23 Online:1996-09-15 Published:1996-09-15
Contact: Jian-Zheng Zhou*

摘要/Abstract

摘要： 本文探讨了鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶 (AC) 活性的影响。鱼油 1.4 ml/kg 每天灌胃两周对大鼠心肌细胞膜上腺苷酸环化酶 (AC) 的基础活性无显著影响, 但可显著地抑制异丙肾上腺素 ( ISO ) 激活的AC 活性。放射配体饱和实验结果显示, 同正常对照相比, 鱼油的摄入对 Bmax 和 Kd 无显著影响, 但同羊油组相比, 可显著地降低β-受体与其配体的亲和力, 同正常对照相比, 羊油的摄入一方面可显著地升高β- 受体与其配体的亲和力, 另一方面又可显著地降低β-受体的数目。结果提示, 鱼油可能主要影响AC 的激活而不是主要影响β-受体本身的功能。

关键词: 鱼油, EPA和DHA, 腺苷酸环化酶, β-受体

Abstract: Effects of fish oil on β-adrenoceptors as well as the activity of adenylate cyclase (AC) on rat myocardial membrane were investigated.Supplementation with fish oil had no significant effect on basal activity of AC on myocardial membrane whereas it could markedly inhibit the AC activity stimulated by isoproterenol (ISO). Radioligand binding assays showed that supplementation with fish oil had no effect on Bmax and Kd, compared with saline control. However, supplementation with sheep oil could markedly reduce both the Kd and Bmax, compared with saline control. And the Kd of sheep oil group was greatly decreased than that of fish oil group. The results suggested that supplementation with fish oil mainly affected the activation of AC, not β-adrenoceptor itself.

Key words: Fish oil, EPA and DHA, Adenylate cyclase, β-adrenoceptor

Supporting:

^*现通讯地址; 北京市军事医学科学院毒物药物研究所(100850)
^*Present address: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850

周建政^*, 吴葆杰, 张岫美. 鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶活性的影响[J]. .

Jian-Zheng Zhou^*, Bao-Jie Wu, Xiu-Mei Zhang . Effect of Fish Oil on β-Adrenoceptors and the Activity of Adenylate Cyclase on Myocardial Membrane in Rats[J]. .