顺铂及其类似物与人红细胞膜磷脂相互作用的研究

顺铂及其类似物与人红细胞膜磷脂相互作用的研究

卢景芬, 夏文胜, 王夔, 翟纯, 刘清亮

1. 北京医科大学天然药物及仿生药物国家重点实验室, 北京 100083;
2. 中国科技大学应用化学系, 2300026;
3. 北京Bruker公司, 1000871

收稿日期:1994-12-12 修回日期:1995-02-10 出版日期:1995-09-15 发布日期:1995-09-15

A Study of Interaction of Cisplatin and Its Analogues withPhospholipid of Erythrocyte Membrane

Jing-Fen Lu, Wen-Sheng Xia, Kui Wang, Chun Zhai, Qing-Liang Liu

1. National Laboratocy of Natural and Biomimetic Drugs; Beijing Medical University; Beijing 100083;
2. Applied Chemistry Department; University of Science and Technology of China; Hefei 230026;
3. Bruker Company, Beijing 100871

Received:1994-12-12 Revised:1995-02-10 Online:1995-09-15 Published:1995-09-15

摘要/Abstract

摘要： 本文采用IR和³¹P NMR方法研究了顺铂及其类似物与人红细胞膜磷脂的相互作用。与对照相比, 顺铂及其类似物加入人红细胞膜以后, 使得红外波谱的1300~953 cm^-1频区发生明显改变。结果分析提示, 顺铂等铂配合物通过静电相互作用和某些配位的方式, 即主要与磷脂的极性头部相互作用。12小时动力学实验进一步表明顺铂的作用是一个可恢复的作用过程, 而其它的铂配合物的作用则不可恢复。³¹P NMR实验得到与IR分析相似的结果。对此进行了讨论。

关键词: 顺铂, 膜磷脂, 31PNMR, IR

Abstract: The interaction of cisplatin and its analogues with phospholipid molecules of human erythrocyte membranes was studied using IR and ³¹P NMR methods. Dramatic changes were ob-served at 1300~953 cm^-1 frequency region on the IR spectra .Based on the IR data analysis, it was speculated that the Pt(II) complexes interacted mainly with the polar head groups of phospholipids through electrostatic interaction and certain coordination patterns. The 12 h dynamic experiment showed that a recoverable process occurred in case of cis-DCDP and an unrecoverable one for other Pt(II) analogues.A similar conclusion could be obtained from ³¹P NMR experimental results. The diversity was discussed.

Key words: Cisplatin, Membrane phospholipid, 31P NMR, IR

Supporting:

卢景芬, 夏文胜, 王夔, 翟纯, 刘清亮. 顺铂及其类似物与人红细胞膜磷脂相互作用的研究[J]. .

Jing-Fen Lu, Wen-Sheng Xia, Kui Wang, Chun Zhai, Qing-Liang Liu. A Study of Interaction of Cisplatin and Its Analogues withPhospholipid of Erythrocyte Membrane[J]. .

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