利福平及其蛋白偶联物致敏兔和利福平特异IgG检测

利福平及其蛋白偶联物致敏兔和利福平特异IgG检测

李林峰, 张孙曦, 李世荫

1. 北京医科大学第三医院皮肤科, 北京 100083;
2. 北京医科大学生物物理教研室, 北京 100083

收稿日期:1993-04-05 修回日期:1993-12-21 出版日期:1994-12-15 发布日期:1994-12-15

The Sensitization Models of Rabbits to Rifampicin and to two kinds of Rifampicin-Protein Conjugates and the Detectionof Rifampicin Specific IgG in these Models

Lin-Feng Li, Sun-Xi Zhang, Shi-Yin Li

1. The Third Hospital, Beijing Medical Universtiy, Beijing, 100083
2. Department of Biophysics, Beijing Medical University, Beijing, 100083

Received:1993-04-05 Revised:1993-12-21 Online:1994-12-15 Published:1994-12-15

摘要/Abstract

摘要： 用利福平及两种利福平-蛋白偶联物:利福平-牛血清白蛋白及利福平-兔血清白蛋白免疫兔, 用ELISA方法对兔抗血清中利福平特异IgG进行检测。利福平-牛血清白蛋白及利福平-兔血清白蛋白的偶联方式及偶联比例不同。利福平特异IgG的效价分别为:利福平免疫组l:l0~1:l00; 利福平-兔血清白蛋白免疫组1:10~l:l000; 利福平-牛血清白蛋白免疫组超过1:1000。抑制实验证明了抗血清的利福平特异性。这些利福平致敏动物模型是首次用利福平或利福平-蛋白偶联物致敏的动物模型。

关键词: 利福平, 动物模型, 变态反应, 酶联免疫吸附实验

Abstract: The Rifampicin (RFP) specific IgG in the sera of rabbits immunized with RFP or with two kinds of RFp-protein conjugates (RFP-bovine serumalbumin, RFP-BSA, and RFP-rabbit serum albumin, RFP-RSA) was measured by ennyme-linked immunosorbent assay (ELISA).The way of conjugation and the molecular cojugation rate were different between RFP-BSdA and RFP-RSA. The titre of the RFP specific IgG was l:l0-l:l00 in RFP immunized rabbits, 1:100-1:1000 in RFP-RSA immunized rabbits and overl:l000 in RFP-BSA immunized rabbits. The binding properties of the sera to RFP were demonstrated by inhibition assay. These are the first RFP sensitization models established by immunizing animals with RFP itself or RFP-protein conjugates.

Key words: Rifampicin, Animalmodel, Hypersensitivity, Enzyme-linked immunosothent assay

Supporting:

李林峰, 张孙曦, 李世荫. 利福平及其蛋白偶联物致敏兔和利福平特异IgG检测[J]. .

Lin-Feng Li, Sun-Xi Zhang, Shi-Yin Li. The Sensitization Models of Rabbits to Rifampicin and to two kinds of Rifampicin-Protein Conjugates and the Detectionof Rifampicin Specific IgG in these Models[J]. .

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